Cytochrome P450s Cyp4p1 and Cyp4p2 associated with the DDT tolerance in the Drosophila melanogaster strain 91-R

Published on Jun 1, 2019in Pesticide Biochemistry and Physiology2.87
· DOI :10.1016/j.pestbp.2019.06.008
Keon Mook Seong5
Estimated H-index: 5
(MSU: Michigan State University),
Brad S. Coates22
Estimated H-index: 22
(ARS: Agricultural Research Service),
Barry Robert Pittendrigh25
Estimated H-index: 25
(MSU: Michigan State University)
Abstract Cytochrome P450s are part of a super-gene family that has undergone gene duplication, divergence, over-expression and, in some cases, loss of function. One such case is the 91-R and 91-C strains of common origin, in Drosophila melanogaster , whereby 91-R (DDT resistant strain) overexpresses Cyp4p1 and Cyp4p2 and both genes are lost in 91-C (DDT susceptible strain). In this study, we used a comparative approach to demonstrate that transcription of Cyp4p1 and Cyp4p2 were constitutively up-regulated in the Drosophila melanogaster strain 91-R as compared to another DDT susceptible strain Canton-S which does not have a loss of function of these genes. Furthermore, significantly increased expression of Cyp4p1 and Cyp4p2 was induced in 91-R in response to sublethal DDT exposure, however, such induction did not occur in the DDT treated Canton-S . Additionally, fixed nucleotide variation within putative transcription factor binding sites of Cyp4p1 and Cyp4p2 promoters were observed between 91-R and Canton-S , however, their impact on transcription remains to be determined. Two GAL4/UAS transgenic strains with integrated heat shock-inducible Cyp4p1 - or Cyp4p2 -RNAi constructs within wild-type genetic backgrounds were developed. Following heat shock induction of Cyp4p1 and Cyp4p2 knockdown, these transgenic lines showed increased DDT mortality as compared to their corresponding non-heat shock controls. These results provide a functional link of Cyp4p1 and Cyp4p2 in conferring tolerance to DDT exposure.
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#1Laura D. Steele (UIUC: University of Illinois at Urbana–Champaign)H-Index: 5
#2Brad S. Coates (USDA: United States Department of Agriculture)H-Index: 22
Last.Barry Robert Pittendrigh (MSU: Michigan State University)H-Index: 25
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#1Ju Hyeon Kim (UMass: University of Massachusetts Amherst)H-Index: 7
#2J.A. Moreau (UMass: University of Massachusetts Amherst)H-Index: 1
Last.John M. Clark (UMass: University of Massachusetts Amherst)H-Index: 23
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#1Aziz Khan (University of Oslo)H-Index: 8
#2Oriol Fornes (UBC: University of British Columbia)H-Index: 9
Last.Ge Tan (Imperial College London)H-Index: 7
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#1Kai Dang (Universiti Sains Malaysia)H-Index: 2
#2Stephen L. Doggett (Westmead Hospital)H-Index: 18
Last.Chow-Yang Lee (Universiti Sains Malaysia)H-Index: 19
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#1Henk van den Berg (WUR: Wageningen University and Research Centre)H-Index: 13
#2Gamini Manuweera (United Nations Environment Programme)H-Index: 7
Last.Flemming Konradsen (UCPH: University of Copenhagen)H-Index: 38
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#1Joshua M. Schmidt (MPG: Max Planck Society)H-Index: 7
#2Paul Battlay (University of Melbourne)H-Index: 5
Last.Charles Robin (University of Melbourne)H-Index: 18
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