Rapid single-step affinity purification of HA-tagged mitochondria from Arabidopsis thaliana

Pages: 672972
Published: Jun 18, 2019
Abstract
null Photosynthesis in plant cells would not be possible without the supportive role of mitochondria. However, isolation of mitochondria from plant cells, for physiological and biochemical analyses, is a lengthy and tedious process. Established isolation protocols require multiple centrifugation steps and substantial amounts of starting material. To overcome these limitations, we tagged mitochondria in Arabidopsis thaliana with a triple...
Paper Details
Title
Rapid single-step affinity purification of HA-tagged mitochondria from Arabidopsis thaliana
Published Date
Jun 18, 2019
Journal
Pages
672972
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