Time-restricted foraging under natural light/dark condition shifts the molecular clock in the honey bee, Apis mellifera

Published on Dec 2, 2018in Chronobiology International2.562
· DOI :10.1080/07420528.2018.1509867
Rikesh Jain2
Estimated H-index: 2
(NCBS: National Centre for Biological Sciences),
Axel Brockmann15
Estimated H-index: 15
(NCBS: National Centre for Biological Sciences)
ABSTRACTHoney bees have a remarkable sense of time and individual honey bee foragers are capable of adjusting their foraging activity with respect to the time of food availability. Although, there is compelling experimental evidence that foraging behavior is guided by the circadian clock, nothing is known about the underlying molecular mechanisms. Here we present for the first time a study that explores whether time-restricted foraging under natural light-dark (LD) condition affects the molecular clock in honey bees. Food was presented in an enclosed flight chamber (12 m × 4 m × 4 m) either for 2 hours in the morning or 2 hours in the afternoon for several consecutive days and daily cycling of the two major clock genes, cryptochrome2 (cry2) and period (per), were analyzed for three different parts of the nervous system involved in feeding-related behaviors: brain, subesophageal ganglion (SEG), and the antennae with olfactory sensory neurons. We found that morning and afternoon trained foragers showed sign...
  • References (58)
  • Citations (1)
📖 Papers frequently viewed together
2 Citations
2 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Aridni Shah (NCBS: National Centre for Biological Sciences)H-Index: 1
#2Rikesh Jain (SASTRA: Shanmugha Arts, Science, Technology & Research Academy)H-Index: 2
Last. Axel Brockmann (NCBS: National Centre for Biological Sciences)H-Index: 15
view all 3 authors...
In honey bees, continuous foraging is accompanied by a sustained up-regulation of the immediate early gene Egr-1 (early growth response protein-1) and candidate downstream genes involved in learning and memory. Here, we present a series of feeder training experiments indicating that Egr-1 expression is highly correlated with the time and duration of training even in the absence of the food reward. Foragers that were trained to visit a feeder over the whole day and then collected on a day without...
4 CitationsSource
#1Alain Dautant (CNRS: Centre national de la recherche scientifique)H-Index: 20
#2Thomas Meier (Imperial College London)H-Index: 31
Last. Roza Kucharczyk (PAN: Polish Academy of Sciences)H-Index: 17
view all 6 authors...
Devastating human neuromuscular disorders have been associated to defects in the ATP synthase. This enzyme is found in the inner mitochondrial membrane and catalyzes the last step in oxidative phosphorylation, which provides aerobic eukaryotes with ATP. With the advent of structures of complete ATP synthases, and the availability of genetically approachable systems such as the yeast Saccharomyces cerevisiae, we can begin to understand these molecular machines and their associated defects at the ...
15 CitationsSource
#1Hongyi Nie (Fujian Agriculture and Forestry University)H-Index: 3
#2Shupeng Xu (Fujian Agriculture and Forestry University)H-Index: 1
Last. Songkun Su (Fujian Agriculture and Forestry University)H-Index: 6
view all 10 authors...
Honey bee is a social insect. Its colony is mainly coordinated by the chemical signals such as pheromones produced by queen or brood. Correspondingly, the worker bee developed numerous complicated olfactory sensilla in antennae for detection of these colony chemical signals and nectar/pollen signals in foraging. With the normal development of new emerged workers, young adults (nurse bee) worked in colony at the first 2–3 weeks and then followed by the foraging activity outside of the hive, which...
5 CitationsSource
#1Moshe Nagari (HUJI: Hebrew University of Jerusalem)H-Index: 3
#2Paul Szyszka (University of Konstanz)H-Index: 16
Last. Guy Bloch (HUJI: Hebrew University of Jerusalem)H-Index: 34
view all 4 authors...
The insect antennae receive olfactory information from the environment. In some insects, it has been shown that antennal responsiveness is dynamically regulated by circadian clocks. However, it is unknown how general this phenomenon is and what functions it serves. Circadian regulation in honeybee workers is particularly interesting in this regard because they show natural task-related chronobiological plasticity. Forager bees show strong circadian rhythms in behavior and brain gene expression, ...
6 CitationsSource
#1Nicholas L. Naeger (UIUC: University of Illinois at Urbana–Champaign)H-Index: 5
#2Gene E. Robinson (UIUC: University of Illinois at Urbana–Champaign)H-Index: 91
ABSTRACT We used transcriptomics to compare instinctive and learned, reward-based honey bee behaviors with similar spatio-temporal components: mating flights by males (drones) and time-trained foraging flights by females (workers), respectively. Genome-wide gene expression profiling via RNA sequencing was performed on the mushroom bodies, a region of the brain known for multi-modal sensory integration and responsive to various types of reward. Differentially expressed genes (DEGs) associated wit...
9 CitationsSource
#1Taro Fuchikawa (Kyoto University)H-Index: 11
#2Ada Eban-Rothschild (Stanford University)H-Index: 12
Last. Guy Bloch (HUJI: Hebrew University of Jerusalem)H-Index: 34
view all 5 authors...
Circadian rhythms in behaviour and physiology are important for animal health and survival. Studies with individually isolated animals in the laboratory have consistently emphasized the dominant role of light for the entrainment of circadian rhythms to relevant environmental cycles. Although in nature interactions with conspecifics are functionally significant, social signals are typically not considered important time-givers for the animal circadian clock. Our results challenge this view. By st...
20 CitationsSource
#1Myles P. Fenske (UW: University of Washington)H-Index: 3
#2Takato Imaizumi (UW: University of Washington)H-Index: 29
To successfully recruit pollinators, plants often release attractive floral scents at specific times of day to coincide with pollinator foraging. This timing of scent emission is thought to be evolutionarily beneficial to maximize resource efficiency while attracting only useful pollinators. Temporal regulation of scent emission is tied to the activity of the specific metabolic pathways responsible for scent production. Although floral volatile profiling in various plants indicated a contributio...
16 CitationsSource
#1Danica F. Patton (SFU: Simon Fraser University)H-Index: 11
#2Ângela M. Katsuyama (UW: University of Washington)H-Index: 2
Last. Ralph E. Mistlberger (SFU: Simon Fraser University)H-Index: 46
view all 13 authors...
Circadian clocks in many brain regions and peripheral tissues are entrained by the daily rhythm of food intake. Clocks in one or more of these locations generate a daily rhythm of locomotor activity that anticipates a regular mealtime. Rats and mice can also anticipate two daily meals. Whether this involves 1 or 2 circadian clocks is unknown. To gain insight into how the circadian system adjusts to 2 daily mealtimes, male rats in a 12∶12 light-dark cycle were fed a 2 h meal either 4 h after ligh...
18 CitationsSource
Following general anesthesia, people are often confused about the time of day and experience sleep disruption and fatigue. It has been hypothesized that these symptoms may be caused by general anesthesia affecting the circadian clock. The circadian clock is fundamental to our well-being because it regulates almost all aspects of our daily biochemistry, physiology, and behavior. Here, we investigated the effects of the most common general anesthetic, isoflurane, on time perception and the circadi...
34 CitationsSource
#1Sandra L. Rodriguez-Zas (UIUC: University of Illinois at Urbana–Champaign)H-Index: 47
#2Bruce R. Southey (UIUC: University of Illinois at Urbana–Champaign)H-Index: 27
Last. Guy Bloch (HUJI: Hebrew University of Jerusalem)H-Index: 34
view all 7 authors...
Honey bee workers care for (“nurse”) the brood around the clock without circadian rhythmicity, but then they forage outside with strong circadian rhythms and a consolidated nightly rest. This chron...
37 CitationsSource
Cited By1
#1Rikesh Jain (AMU: Aix-Marseille University)
#2Axel Brockmann (NCBS: National Centre for Biological Sciences)H-Index: 15
: We performed an RNA-seq based comparison of gene expression levels in the antennae of honey bee drones and time-trained foragers (workers) collected at different times of the day and different activity states. Interestingly, olfaction-related genes (i.e. odorant receptor (Ors), odorant binding proteins (Obps), carboxyl esterases (CEst) etc.) showed stable gene expression differences between drone and worker antennae. Drone antennae showed higher expression of 24 Ors, of which 21 belong to the ...
#1Rikesh Jain (NCBS: National Centre for Biological Sciences)H-Index: 2
#1Rikesh Jain (NCBS: National Centre for Biological Sciences)
Last. Axel Brockmann (NCBS: National Centre for Biological Sciences)H-Index: 15
view all 2 authors...
Eusocial insects, like honey bees, which show an elaborate division of labor involving morphologically and physiologically specialized phenotypes provide a unique toolkit to study molecular underpinnings of behavior as well as neural processing. In this study, we performed an extensive RNA-seq based comparison of gene expression levels in the antennae of honey bee drones and foragers collected at different time of days and activity states to identify molecules involved in peripheral olfactory pr...