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Time-restricted foraging under natural light/dark condition shifts the molecular clock in the honey bee, Apis mellifera

Published on Dec 2, 2018in Chronobiology International2.562
· DOI :10.1080/07420528.2018.1509867
Rikesh Jain2
Estimated H-index: 2
(NCBS: National Centre for Biological Sciences),
Axel Brockmann15
Estimated H-index: 15
(NCBS: National Centre for Biological Sciences)
Abstract
ABSTRACTHoney bees have a remarkable sense of time and individual honey bee foragers are capable of adjusting their foraging activity with respect to the time of food availability. Although, there is compelling experimental evidence that foraging behavior is guided by the circadian clock, nothing is known about the underlying molecular mechanisms. Here we present for the first time a study that explores whether time-restricted foraging under natural light-dark (LD) condition affects the molecular clock in honey bees. Food was presented in an enclosed flight chamber (12 m × 4 m × 4 m) either for 2 hours in the morning or 2 hours in the afternoon for several consecutive days and daily cycling of the two major clock genes, cryptochrome2 (cry2) and period (per), were analyzed for three different parts of the nervous system involved in feeding-related behaviors: brain, subesophageal ganglion (SEG), and the antennae with olfactory sensory neurons. We found that morning and afternoon trained foragers showed sign...
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References58
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#1Aridni Shah (NCBS: National Centre for Biological Sciences)H-Index: 1
#2Rikesh Jain (SASTRA: Shanmugha Arts, Science, Technology & Research Academy)H-Index: 2
Last. Axel Brockmann (NCBS: National Centre for Biological Sciences)H-Index: 15
view all 3 authors...
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#1Alain Dautant (CNRS: Centre national de la recherche scientifique)H-Index: 20
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#2Shupeng Xu (Fujian Agriculture and Forestry University)H-Index: 1
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Honey bee is a social insect. Its colony is mainly coordinated by the chemical signals such as pheromones produced by queen or brood. Correspondingly, the worker bee developed numerous complicated olfactory sensilla in antennae for detection of these colony chemical signals and nectar/pollen signals in foraging. With the normal development of new emerged workers, young adults (nurse bee) worked in colony at the first 2–3 weeks and then followed by the foraging activity outside of the hive, which...
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#1Moshe Nagari (HUJI: Hebrew University of Jerusalem)H-Index: 3
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The insect antennae receive olfactory information from the environment. In some insects, it has been shown that antennal responsiveness is dynamically regulated by circadian clocks. However, it is unknown how general this phenomenon is and what functions it serves. Circadian regulation in honeybee workers is particularly interesting in this regard because they show natural task-related chronobiological plasticity. Forager bees show strong circadian rhythms in behavior and brain gene expression, ...
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#2Gene E. Robinson (UIUC: University of Illinois at Urbana–Champaign)H-Index: 91
ABSTRACT We used transcriptomics to compare instinctive and learned, reward-based honey bee behaviors with similar spatio-temporal components: mating flights by males (drones) and time-trained foraging flights by females (workers), respectively. Genome-wide gene expression profiling via RNA sequencing was performed on the mushroom bodies, a region of the brain known for multi-modal sensory integration and responsive to various types of reward. Differentially expressed genes (DEGs) associated wit...
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Circadian clocks in many brain regions and peripheral tissues are entrained by the daily rhythm of food intake. Clocks in one or more of these locations generate a daily rhythm of locomotor activity that anticipates a regular mealtime. Rats and mice can also anticipate two daily meals. Whether this involves 1 or 2 circadian clocks is unknown. To gain insight into how the circadian system adjusts to 2 daily mealtimes, male rats in a 12∶12 light-dark cycle were fed a 2 h meal either 4 h after ligh...
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Cited By1
Newest
#1Rikesh Jain (AMU: Aix-Marseille University)
#2Axel Brockmann (NCBS: National Centre for Biological Sciences)H-Index: 15
: We performed an RNA-seq based comparison of gene expression levels in the antennae of honey bee drones and time-trained foragers (workers) collected at different times of the day and different activity states. Interestingly, olfaction-related genes (i.e. odorant receptor (Ors), odorant binding proteins (Obps), carboxyl esterases (CEst) etc.) showed stable gene expression differences between drone and worker antennae. Drone antennae showed higher expression of 24 Ors, of which 21 belong to the ...
Source
#1Rikesh Jain (NCBS: National Centre for Biological Sciences)H-Index: 2
#1Rikesh Jain (NCBS: National Centre for Biological Sciences)
Last. Axel Brockmann (NCBS: National Centre for Biological Sciences)H-Index: 15
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Eusocial insects, like honey bees, which show an elaborate division of labor involving morphologically and physiologically specialized phenotypes provide a unique toolkit to study molecular underpinnings of behavior as well as neural processing. In this study, we performed an extensive RNA-seq based comparison of gene expression levels in the antennae of honey bee drones and foragers collected at different time of days and activity states to identify molecules involved in peripheral olfactory pr...
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