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New tools for recombinant protein production in Escherichia coli: A 5-year update: Recombinant protein production in Escherichia coli

Published on Jun 20, 2019in Protein Science 2.42
· DOI :10.1002/pro.3668
Germán L. Rosano8
Estimated H-index: 8
(CONICET: National Scientific and Technical Research Council),
Enrique S. Morales2
Estimated H-index: 2
(CONICET: National Scientific and Technical Research Council),
Eduardo A. Ceccarelli22
Estimated H-index: 22
(CONICET: National Scientific and Technical Research Council)
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Published on Jul 1, 2019in Metabolic Engineering 7.81
Yongkun Lv1
Estimated H-index: 1
(UMBC: University of Maryland, Baltimore County),
Shuai Qian (BCM: Baylor College of Medicine)+ 3 AuthorsPeng Xu35
Estimated H-index: 35
(UMBC: University of Maryland, Baltimore County)
Abstract Metabolic engineering entails target modification of cell metabolism to maximize cell's production potential. Due to the complexity of cell metabolism, feedback genetic circuits have emerged as basic tools to combat metabolic heterogeneity, enhance microbial cooperation as well as boost cell's productivity. This is generally achieved by applying social reward-punishment rules to eliminate cheaters and reward winners in a mixed cell population. With metabolite-responsive transcriptional ...
Published on Apr 1, 2019in Trends in Biotechnology 13.75
Giel Vandemoortele3
Estimated H-index: 3
(UGent: Ghent University),
Sven Eyckerman17
Estimated H-index: 17
(UGent: Ghent University),
Kris Gevaert64
Estimated H-index: 64
(UGent: Ghent University)
Protein tags have been essential for advancing our knowledge of the function of proteins, their localization, and the mapping of their interaction partners. Expressing epitope-tagged proteins has become a standard practice in every life science laboratory and, thus, continues to enable new studies. In recent years, several new tagging moieties have entered the limelight, many of them bringing new functionalities, such as targeted protein degradation, accurate quantification, and proximity labeli...
Published on Mar 1, 2019in Applied Microbiology and Biotechnology 3.67
Thi Khoa My Nguyen2
Estimated H-index: 2
(KU: Korea University),
Mi Ran Ki1
Estimated H-index: 1
(KU: Korea University)
+ 1 AuthorsSeung Pil Pack16
Estimated H-index: 16
(KU: Korea University)
The Escherichia coli (E. coli) expression system has been widely used to produce recombinant proteins. However, in some heterologous expressions, there are still difficulties in large-scale production. The use of fusion partners is one of the strategies for improving the expression levels of proteins in E. coli host. Here, we demonstrate a novel fusion element, the NT11-tag, which enhances protein expression. The NT11-tag was derived from the first 11 amino acid residues within the N-terminal N-...
Published on Feb 1, 2019in Nature Chemical Biology 12.15
Adam J Meyer1
Estimated H-index: 1
(MIT: Massachusetts Institute of Technology),
Thomas H. Segall-Shapiro11
Estimated H-index: 11
(MIT: Massachusetts Institute of Technology)
+ 2 AuthorsChristopher A. Voigt46
Estimated H-index: 46
(MIT: Massachusetts Institute of Technology)
Cellular processes are carried out by many genes, and their study and optimization requires multiple levers by which they can be independently controlled. The most common method is via a genetically encoded sensor that responds to a small molecule. However, these sensors are often suboptimal, exhibiting high background expression and low dynamic range. Further, using multiple sensors in one cell is limited by cross-talk and the taxing of cellular resources. Here, we have developed a directed evo...
Published on Dec 1, 2018in Microbial Cell Factories 4.40
Phillipp Mueller (University of Applied Sciences Biberach), Rahul Gauttam1
Estimated H-index: 1
(University of Ulm)
+ 9 AuthorsBernhard J. Eikmanns54
Estimated H-index: 54
(University of Ulm)
Background Increasing efforts have been made to assess the potential of Escherichia coli strains for the production of complex recombinant proteins. Since a considerable part of therapeutic proteins are glycoproteins, the lack of the post-translational attachment of sugar moieties in standard E. coli expression strains represents a major caveat, thus limiting the use of E. coli based cell factories. The establishment of an E. coli expression system capable of protein glycosylation could potentia...
Published on Dec 1, 2018in Microbial Cell Factories 4.40
Lisa Ann Burdette1
Estimated H-index: 1
(University of California, Berkeley),
Samuel Alexander Leach (NU: Northwestern University)+ 1 AuthorsDanielle Tullman-Ercek15
Estimated H-index: 15
(NU: Northwestern University)
Gram-negative bacteria are attractive hosts for recombinant protein production because they are fast growing, easy to manipulate, and genetically stable in large cultures. However, the utility of these microbes would expand if they also could secrete the product at commercial scales. Secretion of biotechnologically relevant proteins into the extracellular medium increases product purity from cell culture, decreases downstream processing requirements, and reduces overall cost. Thus, researchers a...
Published on Dec 1, 2018in Microbial Cell Factories 4.40
Luminita Gabriela Horga1
Estimated H-index: 1
(University of Manchester),
Samantha Halliwell2
Estimated H-index: 2
(University of Manchester)
+ 8 AuthorsDaniel C. Smith1
Estimated H-index: 1
(Keele University)
Background The secretion of recombinant disulfide-bond containing proteins into the periplasm of Gram-negative bacterial hosts, such as E. coli, has many advantages that can facilitate product isolation, quality and activity. However, the secretion machinery of E. coli has a limited capacity and can become overloaded, leading to cytoplasmic retention of product; which can negatively impact cell viability and biomass accumulation. Fine control over recombinant gene expression offers the potential...
Published on Dec 1, 2018in Nature Communications 11.88
Peter Rugbjerg4
Estimated H-index: 4
(DTU: Technical University of Denmark),
Nils Myling-Petersen2
Estimated H-index: 2
(DTU: Technical University of Denmark)
+ 2 AuthorsMorten Otto Alexander Sommer22
Estimated H-index: 22
(DTU: Technical University of Denmark)
A transition toward sustainable bio-based chemical production is important for green growth. However, productivity and yield frequently decrease as large-scale microbial fermentation progresses, commonly ascribed to phenotypic variation. Yet, given the high metabolic burden and toxicities, evolutionary processes may also constrain bio-based production. We experimentally simulate large-scale fermentation with mevalonic acid-producing Escherichia coli. By tracking growth rate and production, we un...
Published on Nov 16, 2018in ACS Synthetic Biology 5.57
Zoltán Lipinszki10
Estimated H-index: 10
(MTA: Hungarian Academy of Sciences),
Viktor Vernyik2
Estimated H-index: 2
(MTA: Hungarian Academy of Sciences)
+ 5 AuthorsZsuzsanna Gyorfy4
Estimated H-index: 4
(MTA: Hungarian Academy of Sciences)
Escherichia coli is a well-established and popular host for heterologous expression of proteins. The preference in the choice of synonymous codons (codon bias), however, might differ for the host and the original source of the recombinant protein, constituting a potential bottleneck in production. Codon choice affects the efficiency of translation by a complex and poorly understood mechanism. The availability of certain tRNA species is one of the factors that may curtail the capacity of translat...
Published on Oct 1, 2018in Nature Chemical Biology 12.15
Tina Wang5
Estimated H-index: 5
(Broad Institute),
Ahmed H. Badran13
Estimated H-index: 13
(Broad Institute)
+ 1 AuthorsDavid R. Liu69
Estimated H-index: 69
(Broad Institute)
We report the development of soluble expression phage-assisted continuous evolution (SE-PACE), a system for rapidly evolving proteins with increased soluble expression. Through use of a PACE-compatible AND gate that uses a split-intein pIII, SE-PACE enables two simultaneous positive selections to evolve proteins with improved expression while maintaining their desired activities. In as little as three days, SE-PACE evolved several antibody fragments with >5-fold improvement in expression yield w...
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