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A proteome-wide immuno-mass spectrometric identification of serum autoantibodies

Published on Dec 1, 2019in Clinical Proteomics2.89
· DOI :10.1186/s12014-019-9246-0
Milena Music (U of T: University of Toronto), Antoninus Soosaipillai35
Estimated H-index: 35
(MSH: Mount Sinai Hospital, Toronto)
+ 3 AuthorsEleftherios P. Diamandis97
Estimated H-index: 97
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Abstract
Background Autoantibodies are produced when tolerance to self-antigens is broken and they can be mediators of tissue injury and systemic inflammation. They are excellent biomarkers because they are minimally invasive to screen and are highly abundant in serum due to limited proteolysis and slow clearance. Conventionally used methods of identifying autoantibodies in patient sera include indirect immunofluorescence, enzyme-linked immunoabsorbent assays (ELISAs) and protein microarrays. Here we present a novel proteome-wide immuno-mass spectrometric method to identify serum autoantibody targets.
  • References (22)
  • Citations (1)
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References22
Newest
Published on Dec 1, 2018in Journal of Translational Medicine4.10
Michael Gowen2
Estimated H-index: 2
(NYU: New York University),
Keith M. Giles22
Estimated H-index: 22
(NYU: New York University)
+ 11 AuthorsMelissa Wilson9
Estimated H-index: 9
(NYU: New York University)
Background Immune checkpoint inhibitors (anti-CTLA-4, anti-PD-1, or the combination) enhance anti-tumor immune responses, yielding durable clinical benefit in several cancer types, including melanoma. However, a subset of patients experience immune-related adverse events (irAEs), which can be severe and result in treatment termination. To date, no biomarker exists that can predict development of irAEs.
Published on Mar 12, 2018in Frontiers in Immunology4.72
Lucía Sanjurjo8
Estimated H-index: 8
,
Gemma Aran4
Estimated H-index: 4
+ 5 AuthorsMaria-Rosa Sarrias13
Estimated H-index: 13
CD5L (CD5 molecule-like) is a secreted glycoprotein that controls key mechanisms in inflammatory responses, with involvement in processes such as infection, atherosclerosis, and cancer. In macrophages, CD5L promotes an anti-inflammatory cytokine profile in response to TLR activation. In the present study, we questioned whether CD5L is able to influence human macrophage plasticity, and drive its polarization toward any specific phenotype. We compared CD5L-induced phenotypic and functional changes...
Published on Mar 2, 2018in Frontiers in Immunology4.72
Jessica Duarte4
Estimated H-index: 4
(Ludwig Institute for Cancer Research),
Sagun Parakh8
Estimated H-index: 8
(Ludwig Institute for Cancer Research)
+ 7 AuthorsJonathan Cebon59
Estimated H-index: 59
(Ludwig Institute for Cancer Research)
Immune checkpoint inhibitors (ICI) have revolutionized the treatment of advanced melanoma. The first ICI to demonstrate clinical benefit, ipilimumab, targets cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4), however long-term overall survival is just 22%. More than 40 years ago intralesional Bacillus Calmette-Guerin (BCG), a living attenuated strain of Mycobacterium bovis, was found to induce tumor regression by stimulating cell-mediated immunity following a localized and self-limiting infec...
Published on Jan 19, 2018in Frontiers in Immunology4.72
Shanshan Hu2
Estimated H-index: 2
(UTAS: University of Tasmania),
Cameron Marshall1
Estimated H-index: 1
(UTAS: University of Tasmania)
+ 3 AuthorsHeinrich Körner38
Estimated H-index: 38
(UTAS: University of Tasmania)
The absence of tumor necrosis factor (TNF) causes lethal infection by Leishmania major in normally resistant C57BL/6J (B6.WT) mice. The underlying pathogenic mechanism of this fatal disease has so far remained elusive. We found that B6.WT mice deficient for the tnf gene (B6.TNF -/- ) displayed not only a non-healing cutaneous lesion but also a serious infection of the liver upon L. major inoculation. Infected B6.TNF-/- mice developed an enlarged liver that showed increased inflammation. Furtherm...
Published on Dec 1, 2017in Clinical Biochemistry2.43
Sofia Farkona4
Estimated H-index: 4
(U of T: University of Toronto),
Antoninus Soosaipillai35
Estimated H-index: 35
(MSH: Mount Sinai Hospital, Toronto)
+ 5 AuthorsIvan M. Blasutig15
Estimated H-index: 15
(U of T: University of Toronto)
Abstract Background CUB and zona pellucida-like domain-containing protein 1 (CUZD1) was identified as a pancreas-specific protein and was proposed as a candidate biomarker for pancreatic related disorders. CUZD1 protein levels in tissues and biological fluids have not been extensively examined. The purpose of the present study was to generate specific antibodies targeting CUZD1 to assess CUZD1 expression within tissues and biological fluids. Methods Mouse monoclonal antibodies against CUZD1 were...
Published on Mar 1, 2017in Nature Methods28.47
Masaki Matsumoto32
Estimated H-index: 32
,
Fumiko Matsuzaki6
Estimated H-index: 6
+ 13 AuthorsTohru Natsume45
Estimated H-index: 45
(AIST: National Institute of Advanced Industrial Science and Technology)
A large-scale resource, iMPAQT, provides multiple reaction monitoring (MRM)–mass spectrometry assays for targeted quantitative analysis of mTRAQ-labeled human proteins.
Published on Mar 1, 2017in Nature Methods28.47
Daniel Paul Zolg4
Estimated H-index: 4
,
Mathias Wilhelm11
Estimated H-index: 11
+ 21 AuthorsMaximilian Weininger1
Estimated H-index: 1
The ProteomeTools project provides the proteomics community with a physical synthetic tryptic peptide resource and a digital LC-MS/MS data resource covering all human proteins.
Published on Jan 28, 2017in Clinical Chemistry and Laboratory Medicine3.64
Sofia Farkona4
Estimated H-index: 4
(U of T: University of Toronto),
Antoninus Soosaipillai35
Estimated H-index: 35
(U of T: University of Toronto)
+ 4 AuthorsIvan M. Blasutig15
Estimated H-index: 15
(U of T: University of Toronto)
Published on May 1, 2016in Autoimmunity Reviews7.72
Pauline Zaenker2
Estimated H-index: 2
(ECU: Edith Cowan University),
Elin S. Gray29
Estimated H-index: 29
(ECU: Edith Cowan University),
Melanie Ziman24
Estimated H-index: 24
A link between autoimmune responses and cancer via autoantibodies was first described in the 1950s. Since, autoantibodies have been studied for their potential use as cancer biomarkers, however the exact causes of their production remain to be elucidated. This review summarizes current theories of the causes of autoantibody production in cancer, namely: 1) defects in tolerance and inflammation, 2) changes in protein expression levels, 3) altered protein structure, and 4) cellular death mechanism...