Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

Volume: 10, Issue: 1
Published: Jun 11, 2019
Abstract
Cas13d, the type VI-D CRISPR-Cas effector, is an RNA-guided ribonuclease that has been repurposed to edit RNA in a programmable manner. Here we report the detailed structural and functional analysis of the uncultured Ruminococcus sp . Cas13d (UrCas13d)-crRNA complex. Two hydrated Mg 2+ ions aid in stabilizing the conformation of the crRNA repeat region. Sequestration of divalent metal ions does not alter pre-crRNA processing, but abolishes...
Paper Details
Title
Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
Published Date
Jun 11, 2019
Volume
10
Issue
1
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