Application of carboxymethyl cellulose-g-poly(acrylic acid-co-acrylamide) hydrogel sponges for improvement of efficiency, reusability and thermal stability of a recombinant xylanase

Enzyme immobilization onto supports could improve its specificity, storage stability, reusability, pH tolerance and thermal stability. Aiming to provide a proficient immobilization carrier for a recombinant xylanase (PersiXyn1), the high-performance 3D copolymeric network of carboxymethyl cellulose (CMC)-based hydrogel sponges were synthesized using in situ graft radical polymerization. While typical purified gel drying in a vacuum oven made...