A Dual sgRNA Approach for Functional Genomics inArabidopsis thaliana
Abstract
Reverse genetics uses loss-of-function alleles to interrogate gene function. The advent of CRISPR/Cas9-based gene editing now allows the generation of knock-out alleles for any gene and entire gene families. Even in the model plant Arabidopsis thaliana, gene editing is welcomed as T-DNA insertion lines do not always generate null alleles. Here, we show efficient generation of heritable mutations in Arabidopsis using CRISPR/Cas9 with a workload...
Paper Details
Title
A Dual sgRNA Approach for Functional Genomics inArabidopsis thaliana
Published Date
Aug 1, 2018
Journal
Volume
8
Issue
8
Pages
2603 - 2615
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