One-step reverse transcription loop-mediated isothermal amplification: a simple, sensitive and rapid assay for detection of potato virus X in potato leaves and tubers

Volume: 72, Issue: 2, Pages: 321 - 328
Published: Jun 1, 2019
Abstract
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of potato virus X (PVX) targeting coat protein gene. The RT-LAMP assay was carried out using known positive (PVX), LAMP specific primers, amplification at 65 °C for 60 min, 80 °C for 10 min and visualized in agarose gel and viewed directly using SYBR gold staining dye. Change in color from dark orange to fluorescent yellowish green was...
Paper Details
Title
One-step reverse transcription loop-mediated isothermal amplification: a simple, sensitive and rapid assay for detection of potato virus X in potato leaves and tubers
Published Date
Jun 1, 2019
Volume
72
Issue
2
Pages
321 - 328
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