Developing a codon optimization method for improved expression of recombinant proteins in actinobacteria

Codon optimization by synonymous substitution is widely used for recombinant protein expression. Recent studies have investigated sequence features for codon optimization based on large-scale expression analyses. However, these studies have been limited to common host organisms such as Escherichia coli . Here, we develop a codon optimization method for Rhodococcus erythropolis , a gram-positive GC-rich actinobacterium attracting attention as an...