A comparison between whole transcript and 3’ RNA sequencing methods using Kapa and Lexogen library preparation methods

Volume: 20, Issue: 1, Pages: 1 - 12
Published: Jan 7, 2019
Abstract
3’ RNA sequencing provides an alternative to whole transcript analysis. However, we do not know a priori the relative advantage of each method. Thus, a comprehensive comparison between the whole transcript and the 3′ method is needed to determine their relative merits. To this end, we used two commercially available library preparation kits, the KAPA Stranded mRNA-Seq kit (traditional method) and the Lexogen QuantSeq 3’ mRNA-Seq kit (3′ method),...
Paper Details
Title
A comparison between whole transcript and 3’ RNA sequencing methods using Kapa and Lexogen library preparation methods
Published Date
Jan 7, 2019
Volume
20
Issue
1
Pages
1 - 12
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