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Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

Published on Dec 1, 2019in BMC Biotechnology 2.60
· DOI :10.1186/s12896-019-0517-7
Abstract
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References31
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Published on Nov 1, 2018in Nature 41.58
David Cyranoski2
Estimated H-index: 2
,
Heidi Ledford Hkl23
Estimated H-index: 23
The startling announcement by a Chinese scientist represents a controversial leap in the use of genome editing. The startling announcement by a Chinese scientist represents a controversial leap in the use of genome-editing.
16 Citations Source Cite
Published on Oct 23, 2018in PLOS ONE 2.77
Fuminori Tanihara9
Estimated H-index: 9
(University of Tokushima),
Maki Hirata1
Estimated H-index: 1
(University of Tokushima)
+ 6 AuthorsTakeshige Otoi30
Estimated H-index: 30
(University of Tokushima)
TP53 (which encodes p53) is one of the most frequently mutated genes in cancers. In this study, we generated TP53-mutant pigs by gene editing via electroporation of the Cas9 protein (GEEP), a process that involves introducing the Cas9 protein and single-guide RNA (sgRNA) targeting exon 3 and intron 4 of TP53 into in vitro-fertilized zygotes. Zygotes modified by the sgRNAs were transferred to recipients, two of which gave birth to a total of 11 piglets. Of those 11 piglets, 9 survived. Molecular ...
1 Citations Source Cite
Published on Oct 4, 2018in Frontiers in Genetics 4.15
Chao Li4
Estimated H-index: 4
,
Shiwei Zhou4
Estimated H-index: 4
+ 11 AuthorsXingxu Huang14
Estimated H-index: 14
4 Citations Source Cite
Published on Sep 4, 2018in Frontiers in Genetics 4.15
Huaqiang Yang1
Estimated H-index: 1
,
Zhenfang Wu1
Estimated H-index: 1
3 Citations Source Cite
Published on Sep 26, 2017in Journal of Biomedical Research
Ze Li2
Estimated H-index: 2
,
Haiyuan Yang4
Estimated H-index: 4
+ 10 AuthorsYan Liu1
Estimated H-index: 1
4 Citations Source Cite
Published on Aug 1, 2017in Nature 41.58
Hong Ma27
Estimated H-index: 27
(Oregon National Primate Research Center),
Nuria Marti-Gutierrez1
Estimated H-index: 1
(OHSU: Oregon Health & Science University)
+ 28 AuthorsRiffat Ahmed10
Estimated H-index: 10
(OHSU: Oregon Health & Science University)
CRISPR–Cas9 genome editing is used to induce a DNA repair response and correct a disease-causing heterozygous mutation in human embryos with reduced mosaicism and preferential repair using the wild-type copy of the gene.
259 Citations Source Cite
Published on Feb 1, 2017in Scientific Reports 4.12
Ting Zheng1
Estimated H-index: 1
,
Yingzi Hou1
Estimated H-index: 1
+ 14 AuthorsFan Yi9
Estimated H-index: 9
13 Citations Source Cite
Published on Feb 1, 2017in Transgenic Research 2.20
Kristin M. Whitworth22
Estimated H-index: 22
(MU: University of Missouri),
Joshua A. Benne4
Estimated H-index: 4
(MU: University of Missouri)
+ 7 AuthorsKevin D. Wells20
Estimated H-index: 20
(MU: University of Missouri)
The CRISPR/Cas9 genome editing tool has increased the efficiency of creating genetically modified pigs for use as biomedical or agricultural models. The objectives were to determine if DNA editing resulted in a delay in development to the blastocyst stage or in a skewing of the sex ratio. Six DNA templates (gBlocks) that were designed to express guide RNAs that target the transmembrane protease, serine S1, member 2 (TMPRSS2) gene were in vitro transcribed. Pairs of CRISPR guide RNAs that flanked...
9 Citations Source Cite
Published on Jan 1, 2017in Methods of Molecular Biology
Junghyun Ryu5
Estimated H-index: 5
(VT: Virginia Tech),
Kiho Lee6
Estimated H-index: 6
(VT: Virginia Tech)
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Published on Dec 22, 2016in Human Molecular Genetics 4.90
Kai Han2
Estimated H-index: 2
(Southern Medical University),
Liuping Liang2
Estimated H-index: 2
(Southern Medical University)
+ 15 AuthorsFei Jiang2
Estimated H-index: 2
(Guangzhou Institutes of Biomedicine and Health)
Atrichia and sparse hair phenotype cause distress to many patients. Ectodermal dysplasia-9 (ED-9) is a congenital condition characterized by hypotrichosis and nail dystrophy without other disorders, and Hoxc13 is a pathogenic gene for ED-9. However, mice carrying Hoxc13 mutation present several other serious disorders, such as skeletal defects, progressive weight loss and low viability. Mouse models cannot faithfully mimic human ED-9. In this study, we generated an ED-9 pig model via Hoxc13 gene...
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