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Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

Published on Dec 1, 2019in BMC Biotechnology2.30
· DOI :10.1186/s12896-019-0517-7
Kayla Carey1
Estimated H-index: 1
(VT: Virginia Tech),
Junghyun Ryu6
Estimated H-index: 6
(VT: Virginia Tech)
+ 4 AuthorsKiho Lee7
Estimated H-index: 7
(VT: Virginia Tech)
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Abstract
Background The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the efficiency of producing genetically modified animal models and having potential clinical applications as an assisted reproductive technology. Because most of the CRISPR/Cas9 systems introduce site-specific double-stranded breaks (DSBs) to induce site-specific modifications, a major concern is its potential off-targeting activity, which may hinder the application of the technology in clinics. In this study, we investigated off-targeting events in genome edited pigs/fetuses that were generated through direct injection of the CRISPR/Cas9 system into developing embryos; off-targeting activity of four different sgRNAs targeting RAG2, IL2RG, SCD5, and Ig Heavy chain were examined.
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References31
Newest
40 CitationsSource
#1Fuminori Tanihara (University of Tokushima)H-Index: 9
#2Maki Hirata (University of Tokushima)H-Index: 3
Last.Takeshige Otoi (University of Tokushima)H-Index: 30
view all 9 authors...
5 CitationsSource
#1Chao Li (NWAFU: Northwest A&F University)H-Index: 5
#2Shiwei Zhou (NWAFU: Northwest A&F University)H-Index: 6
Last.Xiaolong Wang (NWAFU: Northwest A&F University)H-Index: 10
view all 14 authors...
9 CitationsSource
#1Huaqiang Yang (SCAU: South China Agricultural University)H-Index: 1
#2Zhenfang Wu (SCAU: South China Agricultural University)H-Index: 14
9 CitationsSource
#1Hong Ma (Oregon National Primate Research Center)H-Index: 27
#2Nuria Marti-Gutierrez (OHSU: Oregon Health & Science University)H-Index: 1
Last.Shoukhrat MitalipovH-Index: 36
view all 31 authors...
328 CitationsSource
#1Ting Zheng (PKU: Peking University)H-Index: 1
#2Yingzi Hou (PKU: Peking University)H-Index: 1
Last.Quan Du (PKU: Peking University)H-Index: 28
view all 17 authors...
21 CitationsSource
#1Kristin M. Whitworth (MU: University of Missouri)H-Index: 22
#2Joshua A. Benne (MU: University of Missouri)H-Index: 4
Last.Kevin D. Wells (MU: University of Missouri)H-Index: 30
view all 10 authors...
12 CitationsSource
#1Ze LiH-Index: 3
#2Haiyuan YangH-Index: 4
Last.Yifan DaiH-Index: 20
view all 13 authors...
7 CitationsSource
#1Junghyun Ryu (VT: Virginia Tech)H-Index: 6
#2Kiho Lee (VT: Virginia Tech)H-Index: 7
2 CitationsSource
#1Kai Han (Southern Medical University)H-Index: 2
#2Liuping Liang (Southern Medical University)H-Index: 2
Last.Nana Fan (Guangzhou Institutes of Biomedicine and Health)H-Index: 14
view all 18 authors...
8 CitationsSource
Cited By1
Newest
#1Marie R. Mooney (Duke University)H-Index: 1
#2Erica E. Davis (Duke University)H-Index: 38
Last.Nicholas Katsanis (Duke University)H-Index: 79
view all 3 authors...
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