Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection

CRISPR/Cas9 mediated gene knockout is a powerful tool for genome editing with the ability to target multiple genes simultaneously. Establishing an efficient, multiplexed gene knockout system using CRISPR/Cas9 that is both simple and robust in its application would further advance the adoption of CRISPR/Cas9 for genetic studies.In this study, we present a simple, versatile and highly efficient method to achieve acute gene knockout with...