Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae

Metabolic resistance of the major malaria vector Anopheles gambiae (s.l.) to insecticides is operationally significant, particularly in combination with target site resistance. However, detection of metabolic resistance is not trivial and relies on laborious bioassays, unspecific biochemical methods, or sophisticated and expensive molecular approaches using transcriptomics. Rapid one-step multiplex TaqMan-probe based RT-qPCR assays were...