Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system-based genome editing has been applied to a wide range of species, including economically important agricultural crops (Doudna and Charpentier, 2014). Genome editing by the direct delivery of ribonucleoproteins (RNPs) offers various advantages compared to the conventional transgene-based method, including high efficiency, low time...