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Evaluation of treatment of experimentally induced canine model of multiple sclerosis using laser activated non-expanded adipose derived stem cells

Published on May 1, 2019in Research in Veterinary Science 1.75
· DOI :10.1016/j.rvsc.2019.05.016
Ahmed N. Abdallah1
Estimated H-index: 1
,
Ashraf Shamaa4
Estimated H-index: 4
,
Omar S. El-Tookhy3
Estimated H-index: 3
Cite
Abstract
Abstract Multiple sclerosis (MS) is a progressive demyelinating disease of the central nervous system that destroys oligodendrocytes. This work aims to evaluate the treatment of experimentally induced MS in dogs using laser activated non-expanded adipose derived stem cells. The results showed amelioration of the clinical signs over time confirmed by the resolution of the previous lesions on MRI. Positive migration of the injected cells to the site of lesion, increased remyelination detected by Myelin Basic Proteins, positive differentiation into Olig2 positive oligodendrocytes, prevented the glial scar formation and restored axonal architecture. The study concluded that treatment using laser activated stem cells holds a promising therapeutic option for treatment of MS in a canine model.
  • References (47)
  • Citations (0)
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References47
Newest
Published on Dec 1, 2018in Scientific Reports 4.01
Alessia Farinazzo15
Estimated H-index: 15
(University of Verona),
Stefano Angiari12
Estimated H-index: 12
(University of Verona)
+ 7 AuthorsBruno Bonetti30
Estimated H-index: 30
(University of Verona)
Cell based-therapies represent promising strategies for the treatment of neurological diseases. We have previously shown that adipose stem cells (ASC) ameliorate chronic experimental autoimmune encephalomyelitis (EAE). Recent evidence indicates that most ASC paracrine effects are mediated by extracellular vesicles, i.e. micro- and nanovesicles (MVs and NVs). We show that preventive intravenous administration of NVs isolated from ASC (ASC-NVs) before disease onset significantly reduces the severi...
Published on Dec 1, 2016in Stem Cell Research & Therapy 4.63
Johannes Leibacher2
Estimated H-index: 2
(Goethe University Frankfurt),
Reinhard Henschler31
Estimated H-index: 31
(Goethe University Frankfurt)
Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. They were found to be potent in situations such as tissue repair or severe inflammation. Still, data are lacking with regard to the biodistribution of MSCs, their cellular or molecular target structures, and the mechanisms by which MSCs reach these targets. This review discusses current hypotheses for how MSCs can reach tissue sites. Both preclinical and clinical studies using MSCs appli...
Published on Aug 1, 2016in Clinical Immunology 3.55
Adel Mohammadzadeh4
Estimated H-index: 4
(Urmia University of Medical Sciences),
A A Pourfath Elah18
Estimated H-index: 18
(TMU: Tarbiat Modares University)
+ 5 AuthorsMasoud Soleimani36
Estimated H-index: 36
(TMU: Tarbiat Modares University)
Abstract Interferon-β (IFN-β) is commonly used as a disease modifying drug for the treatment of relapse-remitting multiple sclerosis (RR-MS). However, the underlying mechanism by which IFN-β mediate this immunosuppressive effect is still unknown. In this study, we analyzed the effects of genetically modified adipose-derived mesenchymal stem cells (AD-MSCs) expressing murine interferon beta (MSCs-VP/IFN-β) on the animal model of MS, experimental autoimmune encephalomyelitis (EAE). Lymph node mono...
Published on Jan 1, 2016in Advances in Bioscience and Biotechnology
Moataz A. Elawady2
Estimated H-index: 2
,
Mohammed M. Elmaghrabi2
Estimated H-index: 2
+ 4 AuthorsAlyaa Ragaei1
Estimated H-index: 1
Background: Spinal cord injury (SCI) unsuccessful regeneration was due to glial scar development. It was a major obstacle to axonal restoration. Safe therapeutic intervention by the use of bone marrow derived stem cells (BMMSCs) transplantation applied in the present study could reduce spinal disability. Material and methods: Forty male albino rats were divided into four groups: GI: negative control (n = 10 rats); GII: positive control after SCI (n = 10 rats); GIII: SCI + BM - MSCs intravenous i...
Published on Sep 1, 2015in Cell Transplantation 3.48
Hala Gabr9
Estimated H-index: 9
(Cairo University),
Wael Abo Elkheir3
Estimated H-index: 3
(Military Medical Academy)
+ 5 AuthorsHatem E. Sabaawy12
Estimated H-index: 12
(RU: Rutgers University)
Spinal cord injury (SCI) results in demyelination of surviving axons, loss of oligodendrocytes, and impairment of motor and sensory functions. We have developed a clinical strategy of cell therapy for SCI through the use of autologous bone marrow cells for transplantation to augment remyelination and enhance neurological repair. In a preclinical large mammalian model of SCI, experimental dogs were subjected to a clipping contusion of the spinal cord. Two weeks after the injury, GFP-labeled autol...
Published on Jan 1, 2015in Brain 11.81
Amélie Wegener2
Estimated H-index: 2
,
Cyrille Deboux10
Estimated H-index: 10
+ 6 AuthorsBrahim Nait-Oumesmar23
Estimated H-index: 23
The basic helix-loop-helix transcription factor Olig2 is a key determinant for the specification of neural precursor cells into oligodendrocyte progenitor cells. However, the functional role of Olig2 in oligodendrocyte migration and differentiation remains elusive both during developmental myelination and under demyelinating conditions of the adult central nervous system. To decipher Olig2 functions, we generated transgenic mice (TetOlig2:Sox10rtTA/+) overexpressing Olig2 in Sox10+ oligodendrogl...
Published on Oct 1, 2014in Journal of Neuroimmunology 2.83
Azim Hedayatpour4
Estimated H-index: 4
(Tehran University of Medical Sciences),
Iraj Ragerdi2
Estimated H-index: 2
(Tehran University of Medical Sciences)
+ 1 AuthorsLaya Kafami5
Estimated H-index: 5
Published on May 1, 2014in Molecular Biotechnology 1.71
Nazem Ghasemi2
Estimated H-index: 2
(IUMS: Isfahan University of Medical Sciences),
Shahnaz Razavi17
Estimated H-index: 17
(IUMS: Isfahan University of Medical Sciences)
+ 3 AuthorsSayyed Hamid Zarkesh Esfahani5
Estimated H-index: 5
(UI: University of Isfahan)
Adipose-derived stem cells (ADSCs) are a desirable stem cell source in neurodegenerative diseases treatment due to their ability to differentiate into different cell lineages. In this study, we transplanted human ADSCs (hADSCs) into a lysophosphatidylcholine (lysolecithin) model of multiple sclerosis (MS) and determined the efficiency of these cells in remyelination process. Forty adult rats were randomly divided into control, lysolecithin, vehicle, and transplantation groups, and focal demyelin...
Published on Jan 1, 2014in BMC Veterinary Research 1.79
Natasha J. Olby31
Estimated H-index: 31
(NCSU: North Carolina State University),
Ji-Hey Lim8
Estimated H-index: 8
(NCSU: North Carolina State University)
+ 6 AuthorsAudrey C. Muguet-Chanoit5
Estimated H-index: 5
(NCSU: North Carolina State University)
Background An inexpensive method of generating continuous data on hind limb function in dogs with spinal cord injury is needed to facilitate multicentre clinical trials. This study aimed to define normal fore limb, hind limb coordination in dogs walking on a treadmill and then to determine whether reliable data could be generated on the frequency of hind limb stepping and the frequency of coordinated stepping in dogs with a wide range of severities of thoracolumbar spinal cord injury.
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Newest
Published on Jul 20, 2019in Molecular Biology Reports 2.11
Christopher M. Duma9
Estimated H-index: 9
,
Oleg V. Kopyov9
Estimated H-index: 9
+ 20 AuthorsChad A. Caraway2
Estimated H-index: 2
(UCI: University of California, Irvine)
We have chosen to test the safety of human intracerebroventricular (ICV) brain injections of autologous non-genetically-modified adipose-derived stromal vascular fraction (ADSVF). In this IRB-approved trial, 24 patients received ICV ADSVF via an implanted reservoir between 5/22/14 and 5/22/17. Seven others were injected via their ventriculo-peritoneal shunts. Ten patients had Alzheimer’s disease (AD), 6 had amyotrophic lateral sclerosis (ALS), 6 had progressive multiple sclerosis (MS-P), 6 had P...