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Analysis of neurofilament concentration in healthy adult horses and utility in the diagnosis of equine protozoal myeloencephalitis and equine motor neuron disease

Published on Aug 1, 2019in Research in Veterinary Science 1.75
· DOI :10.1016/j.rvsc.2019.04.018
Adriana M. Morales Gómez , Sophie Zhu + 5 AuthorsHussni O. Mohammed38
Estimated H-index: 38
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Abstract
Abstract Neurofilaments (NFs) are structural proteins of neurons that are released in significant quantities in the cerebrospinal fluid and blood as a result of neuronal degeneration or axonal damage. Therefore, NFs have potential as biomarkers for neurologic disorders. Neural degeneration increases with age and has the potential to confound the utility of NFs as biomarkers in the diagnosis of neurologic disorders. We investigated this relationship in horses with and without neurological diagnosis. While controlling for horse type (draft, pleasure, and racing), we evaluated the relationship between serum heavy-chain phosphorylated neurofilaments (pNF-H) and age, sex, and serum vitamin E concentrations. Serum pNF-H concentrations increased by 0.002 ng/ml for each year increase in age. There were significant differences in the serum pNF-H concentration among the type of activity performed by the horse. The highest serum pNF-H concentration was found in horses performing heavy work activity (racehorse) and with lower serum pNF-H concentration found among light (pleasure riding) and moderate (draft) activity. There was no significant association between the pNF-H concentration and sex or vitamin E concentration. Serum pNF-H concentration was elevated among horses afflicted with EMND and EPM when compared with control horses without evidence of neurologic disorders. Accordingly, serum pNF-H concentration can serve as a useful biomarker to complement the existing diagnostic work-up of horses suspected of having EPM or EMND.
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References40
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Published on Nov 1, 2017in Equine Veterinary Journal 2.12
J. C. Brown1
Estimated H-index: 1
(UMN: University of Minnesota),
Stephanie J. Valberg4
Estimated H-index: 4
(MSU: Michigan State University)
+ 1 AuthorsCarrie J. Finno13
Estimated H-index: 13
(UC Davis: University of California, Davis)
SummaryBackground Alpha-tocopherol (α-TP) supplementation is recommended for the prevention of various equine neuromuscular disorders. Formulations available include RRR-α-TP acetate powder and a more expensive but rapidly water-dispersible liquid RRR-α-TP (WD RRR-α-TP). No cost-effective means of rapidly increasing serum and cerebrospinal fluid (CSF) α-TP with WD RRR-α-TP and then sustaining concentrations with RRR-α-TP acetate has yet been reported. Objectives To evaluate serum, CSF and muscle...
Published on Oct 1, 2017in Research in Veterinary Science 1.75
A.R. Intan-Shameha1
Estimated H-index: 1
(UPM: Universiti Putra Malaysia),
Thomas J. Divers32
Estimated H-index: 32
(Cornell University)
+ 4 AuthorsHussni O. Mohammed38
Estimated H-index: 38
(Cornell University)
Abstract The current study aimed at the investigating the potential use of phosphorylated neurofilament H (pNF-H) as a diagnostic biomarker for neurologic disorders in the horse. Paired serum and cerebrospinal fluid (CSF) samples ( n = 88) and serum only ( n = 30) were obtained from horses diagnosed with neurologic disorders and clinically healthy horses as control. The neurologic horses consisted of equine protozoal myeloencephalitis (EPM) (38 cases) and cervical vertebral malformation (CVM) (2...
Published on Aug 3, 2017in Expert Review of Molecular Diagnostics 3.10
Aylin Yilmaz14
Estimated H-index: 14
(University of Gothenburg),
Kaj Blennow118
Estimated H-index: 118
(Sahlgrenska University Hospital)
+ 7 AuthorsMagnus Gisslén38
Estimated H-index: 38
(University of Gothenburg)
Introduction: Several CSF biomarkers of neuronal injury have been studied in people living with HIV. At this time, the most useful is the light subunit of the neurofilament protein (NFL). This major structural component of myelinated axons is essential to maintain axonal caliber and to facilitate effective nerve conduction. CSF concentrations of NFL provide a sensitive marker of CNS injury in a number of neurological diseases, including HIV-related neuronal injury. Areas Covered: In this review,...
Published on Jul 1, 2017in Annals of Neurology 9.50
Tania F. Gendron20
Estimated H-index: 20
(Mayo Clinic),
Lillian M. Daughrity15
Estimated H-index: 15
+ 16 AuthorsJames D. Berry21
Estimated H-index: 21
As potential treatments for C9ORF72-associated amyotrophic lateral sclerosis (c9ALS) approach clinical trials, the identification of prognostic biomarkers for c9ALS becomes a priority. We show that levels of phosphorylated neurofilament heavy chain (pNFH) in cerebrospinal fluid (CSF) predict disease status and survival in c9ALS patients, and are largely stable over time. Moreover, c9ALS patients exhibit higher pNFH levels, more rapid disease progression, and shorter survival after disease onset ...
Published on Jul 1, 2017in Neuroscience Letters 2.17
Reo Inoue2
Estimated H-index: 2
(UTokyo: University of Tokyo),
Masahiko Sumitani14
Estimated H-index: 14
(UTokyo: University of Tokyo)
+ 6 AuthorsYoshitsugu Yamada18
Estimated H-index: 18
(UTokyo: University of Tokyo)
Abstract Introduction Approximately 50–80% patients experience postoperative delirium, an acute cognitive dysfunction associated with prolonged hospitalization, increased mortality, excess healthcare costs, and persistent cognitive impairment. Elucidation of the mechanism of delirium and associated diagnostic and therapeutic measures are urgently required. Here we investigated the role of phosphorylated neurofilament heavy subunit (pNF-H), a major structural protein in axons, as a predictive mak...
Published on Jul 1, 2017in American Journal of Physiology-cell Physiology 3.55
Henrik Zetterberg84
Estimated H-index: 84
(University of Gothenburg)
Alzheimer9s disease (AD) is a common neurodegenerative disease that starts with a clinically silent phase of a decade or more during which brain pathologies accumulate predominantly in the medial temporal lobe but also elsewhere in the brain. Network dysfunction and clinical symptoms typically appear when senile plaque (amyloid β) and neurofibrillary tangle (tau) pathologies meet in the brain parenchyma, producing synapse and neuronal loss. For plaque and tangle pathologies, reliable fluid bioma...
Published on Jul 1, 2017in Veterinary Ophthalmology 1.02
Carrie J. Finno13
Estimated H-index: 13
(UC Davis: University of California, Davis),
Heather J. Kaese5
Estimated H-index: 5
+ 3 AuthorsStephanie J. Valberg4
Estimated H-index: 4
(MSU: Michigan State University)
Objective A pigment retinopathy has been reported in adult horses with equine motor neuron disease (EMND) arising from chronic α-tocopherol (α-TP) deficiency. A pigment retinopathy has not been identified in horses with neuroaxonal dystrophy/equine degenerative myeloencephalopathy (NAD/EDM) that affects genetically susceptible young horses with α-TP deficiency. The objective of this report is to describe, for the first time, a pigment retinopathy in a family of α-TP-deficient Warmbloods (WB) wit...
Published on Jun 13, 2017in Neurology 8.69
Koen Poesen16
Estimated H-index: 16
,
Maxim De Schaepdryver2
Estimated H-index: 2
+ 24 AuthorsOlivier Stevens3
Estimated H-index: 3
Objective: To determine the diagnostic performance and prognostic value of phosphorylated neurofilament heavy chain (pNfH) and neurofilament light chain (NfL) in CSF as possible biomarkers for amyotrophic lateral sclerosis (ALS) at the diagnostic phase. Methods: We measured CSF pNfH and NfL concentrations in 220 patients with ALS, 316 neurologic disease controls (DC), and 50 genuine disease mimics (DM) to determine and assess the accuracy of the diagnostic cutoff value for pNfH and NfL and to co...
Published on Jun 1, 2017in Annals of Neurology 9.50
Giulio Disanto24
Estimated H-index: 24
,
Christian Barro12
Estimated H-index: 12
+ 10 AuthorsDavid Leppert36
Estimated H-index: 36
OBJECTIVE Neurofilament light chains (NfL) are unique to neuronal cells, are shed to the cerebrospinal fluid (CSF), and are detectable at low concentrations in peripheral blood. Various diseases causing neuronal damage have resulted in elevated CSF concentrations. We explored the value of an ultrasensitive single-molecule array (Simoa) serum NfL (sNfL) assay in multiple sclerosis (MS). METHODS sNfL levels were measured in healthy controls (HC, n = 254) and two independent MS cohorts: (1) cross-s...
Published on May 9, 2017in Neurology 8.69
Pashtun Shahim10
Estimated H-index: 10
,
Henrik Zetterberg84
Estimated H-index: 84
+ 1 AuthorsKaj Blennow118
Estimated H-index: 118
Objective: To evaluate whether the axonal protein neurofilament light (NFL) in serum is a sensitive biomarker to detect subtle brain injury or concussion in contact sports athletes.Methods: Two pro ...
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