Optimization of protein entrapment in affinity microcolumns using hydrazide-activated silica and glycogen as a capping agent
Abstract
Several approaches were compared for the entrapment of proteins within hydrazide-activated silica for use in affinity microcolumns and high performance affinity chromatography. Human serum albumin (HSA) and concanavalin A (Con A) were used as model proteins for this work. Items considered in this study included the role played by the solution volume, amount of added protein, and use of slurry vs. on-column entrapment on the levels of solute...
Paper Details
Title
Optimization of protein entrapment in affinity microcolumns using hydrazide-activated silica and glycogen as a capping agent
Published Date
Jul 1, 2019
Journal
Volume
1121
Pages
1 - 8
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