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Two functionally distinct CYP4G genes of Anopheles gambiae contribute to cuticular hydrocarbon biosynthesis

Published on Jul 1, 2019in Insect Biochemistry and Molecular Biology3.62
· DOI :10.1016/j.ibmb.2019.04.018
Mary Kefi1
Estimated H-index: 1
,
Vasileia Balabanidou4
Estimated H-index: 4
+ 3 AuthorsJohn Vontas39
Estimated H-index: 39
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Abstract
Abstract Cuticular hydrocarbon (CHC) biosynthesis is a major pathway of insect physiology. In Drosophila melanogaster the cytochrome P450 CYP4G1 catalyses the insect-specific oxidative decarbonylation step, while in the malaria vector Anopheles gambiae , two CYP4G paralogues, CYP4G16 and CYP4G17 are present. Analysis of the subcellular localization of CYP4G17 and CYP4G16 in larval and pupal stages revealed that CYP4G16 preserves its PM localization across developmental stages analyzed; however CYPG17 is differentially localized in two distinct types of pupal oenocytes, presumably oenocytes of larval and adult developmental specificity. Western blot analysis showed the presence of two CYP4G17 forms, potentially associated with each oenocyte type. Both An. gambiae CYP4G s were expressed in D. melanogaster flies in a Cyp4g1 silenced background in order to functionally characterize them in vivo . CYP4G16 , CYP4G17 or their combination rescued the lethal phenotype of Cyp4g1- knock down flies, demonstrating that CYP4G17 is also a functional decarbonylase, albeit of somewhat lower efficiency than CYP4G16 in Drosophila . Flies expressing mosquito CYP4G16 and/or CYP4G17 produced similar CHC profiles to ‘wild-type’ flies expressing the endogenous CYP4G1, but they also produce very long-chain dimethyl-branched CHCs not detectable in wild type flies, suggesting that the specificity of the CYP4G enzymes contributes to determine the complexity of the CHC blend. In conclusion, both An. gambiae CYP4G enzymes contribute to the unique Anopheles CHC profile, which has been associated to defense, adult desiccation tolerance, insecticide penetration rate and chemical communication.
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References34
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#2Jeffrey Nadeau (UNR: University of Nevada, Reno)H-Index: 3
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#1Bernarda Calla (UIUC: University of Illinois at Urbana–Champaign)H-Index: 11
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Last.May R. Berenbaum (UIUC: University of Illinois at Urbana–Champaign)H-Index: 67
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#1Vasileia Balabanidou (FORTH: Foundation for Research & Technology – Hellas)H-Index: 4
#2Linda Grigoraki (UoC: University of Crete)H-Index: 6
Last.John Vontas (FORTH: Foundation for Research & Technology – Hellas)H-Index: 39
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#1Vasileia Balabanidou (FORTH: Foundation for Research & Technology – Hellas)H-Index: 4
#2Anastasia Kampouraki (UoC: University of Crete)H-Index: 4
Last.Amy Lynd (LSTM: Liverpool School of Tropical Medicine)H-Index: 11
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#2Diana L. Huestis (NIH: National Institutes of Health)H-Index: 12
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#2Damien Garrido (University of Paris-Sud)H-Index: 3
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#1Henry Chung (HHMI: Howard Hughes Medical Institute)H-Index: 13
#2David W. Loehlin (HHMI: Howard Hughes Medical Institute)H-Index: 2
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