Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing-based quantification
Abstract
Quantification of DNA sequence tags from engineered constructs such as plasmids, transposons, or other transgenes underlies many functional genomics measurements. Typically, such measurements rely on PCR followed by next-generation sequencing. However, PCR amplification can introduce significant quantitative error. We describe REcount, a novel PCR-free direct counting method. Comparing measurements of defined plasmid pools to droplet digital PCR...
Paper Details
Title
Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing-based quantification
Published Date
Apr 29, 2019
Journal
Volume
20
Issue
1
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