Sequence capture across large phylogenetic scales by using pooled PCR‐generated baits: A case study of Lepidoptera

Volume: 19, Issue: 4, Pages: 1037 - 1051
Published: Jun 1, 2019
Abstract
Sequence capture across large phylogenetic scales is not easy because hybridization capture is only effective when the genetic distance between the bait and target is small. Here, we propose a simple but effective strategy to tackle this issue: pooling DNA from a number of selected representative species of different clades to prepare PCR-generated baits to minimize the genetic distance between the bait and target. To demonstrate the utility of...
Paper Details
Title
Sequence capture across large phylogenetic scales by using pooled PCR‐generated baits: A case study of Lepidoptera
Published Date
Jun 1, 2019
Volume
19
Issue
4
Pages
1037 - 1051
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