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The Impact of Pyroglutamate: Sulfolobus acidocaldarius Has a Growth Advantage over Saccharolobus solfataricus in Glutamate-Containing Media

Published on Apr 24, 2019in Archaea3.09
· DOI :10.1155/2019/3208051
Anna M. Vetter (Braunschweig University of Technology), Julia Helmecke (Braunschweig University of Technology)+ 1 AuthorsMeina Neumann-Schaal5
Estimated H-index: 5
(Braunschweig University of Technology)
Abstract
Microorganisms are well adapted to their habitat but are partially sensitive to toxic metabolites or abiotic compounds secreted by other organisms or chemically formed under the respective environmental conditions. Thermoacidophiles are challenged by pyroglutamate, a lactam that is spontaneously formed by cyclization of glutamate under aerobic thermoacidophilic conditions. It is known that growth of the thermoacidophilic crenarchaeon Saccharolobus solfataricus (formerly Sulfolobus solfataricus) is completely inhibited by pyroglutamate. In the present study, we investigated the effect of pyroglutamate on the growth of S. solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. In contrast to S. solfataricus, S. acidocaldarius was successfully cultivated with pyroglutamate as a sole carbon source. Bioinformatical analyses showed that both members of the Sulfolobaceae have at least one candidate for a 5-oxoprolinase, which catalyses the ATP-dependent conversion of pyroglutamate to glutamate. In S. solfataricus, we observed the intracellular accumulation of pyroglutamate and crude cell extract assays showed a less effective degradation of pyroglutamate. Apparently, S. acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to S. solfataricus. Concludingly, S. acidocaldarius exhibits a more efficient utilization of pyroglutamate and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures.
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References27
Newest
#1Jin Sup Park (PNU: Pusan National University)H-Index: 7
#2Areum Lee (PNU: Pusan National University)H-Index: 3
Last.Jaeho Cha (PNU: Pusan National University)H-Index: 19
view all 6 authors...
#1Helge Stark (Braunschweig University of Technology)H-Index: 2
#2Jacqueline Wolf (Braunschweig University of Technology)H-Index: 2
Last.Dietmar Schomburg (Braunschweig University of Technology)H-Index: 57
view all 10 authors...
#1Sandra Placzek (Braunschweig University of Technology)H-Index: 5
#2Ida Schomburg (Braunschweig University of Technology)H-Index: 10
Last.Dietmar Schomburg (Braunschweig University of Technology)H-Index: 57
view all 7 authors...
#1Ofir Cohen (Weizmann Institute of Science)H-Index: 15
#2Shany Doron (Weizmann Institute of Science)H-Index: 9
Last.Rotem Sorek (Weizmann Institute of Science)H-Index: 46
view all 8 authors...
#1Lex Overmars (Netherlands Bioinformatics Centre)H-Index: 11
#2Robert Kerkhoven (Radboud University Nijmegen Medical Centre)H-Index: 1
Last.Christof FranckeH-Index: 15
view all 4 authors...
#1Michaela Wagner (MPG: Max Planck Society)H-Index: 6
#2Marleen van Wolferen (MPG: Max Planck Society)H-Index: 11
Last.Sonja-Verena Albers (MPG: Max Planck Society)H-Index: 42
view all 7 authors...
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