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Structural characterization and immunostimulatory activity of a novel polysaccharide isolated with subcritical water from Sagittaria sagittifolia L.

Published on Jul 1, 2019in International Journal of Biological Macromolecules4.78
· DOI :10.1016/j.ijbiomac.2019.04.077
Jixian Zhang6
Estimated H-index: 6
(Jiangsu University),
Meng Chen3
Estimated H-index: 3
(Jiangsu University)
+ 6 AuthorsHaile Ma19
Estimated H-index: 19
(Jiangsu University)
Abstract
Abstract In the present study, we obtained polysaccharides from Sagittaria sagittifolia L. (SSP) with subcritical water extraction (SWE). Two water-soluble polysaccharides (SSP-W1 and SSP-S1) from the acquired SSP were isolated with DEAE-52 and Sephadex G-100. Besides, the structural characteristics and immunostimulatory activity were also investigated. The results showed that both SSP-W1 and SSP-S1 were homogeneous polysaccharides and the molecular weight was 62.03 KDa and 15.2 KDa, respectively. In addition, both SSP-W1 and SSP-S1 are heteropolysaccharides. Moreover, FT-IR analysis showed that SSP-W1 was α-pyranose polysaccharide, while SSP-S1 was a typical β-pyranose polysaccharide. Congo red staining showed that there was no triple helix structure in both SSP-W1 and SSP-S1. Furthermore, both SSP-W1 and SSP-S1 could promote the proliferation, production of NO, and secretion of TNF-α and IL-10 of macrophages RAW 264.7, significantly. Therefore, the polysaccharides extracted from Sagittaria sagittifolia L. with SWE have the potential to be used as immunoreactive agent in medicine and functional foods.
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  • Citations (4)
References86
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#1Jixian Zhang (Jiangsu University)H-Index: 6
#2Chaoting Wen (Jiangsu University)H-Index: 6
Last.Haile Ma (Jiangsu University)H-Index: 19
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5 CitationsSource
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#2Jixian Zhang (Jiangsu University)H-Index: 6
Last.Haile Ma (Jiangsu University)H-Index: 19
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#1Yeon Jin Cho (Pukyong National University)H-Index: 3
#2Adane Tilahun Getachew (AAU: Addis Ababa University)H-Index: 7
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#1Jixian Zhang (Jiangsu University)H-Index: 6
#2Chaoting Wen (Jiangsu University)H-Index: 6
Last.Haile Ma (Jiangsu University)H-Index: 19
view all 6 authors...
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#2Chaoting Wen (Jiangsu University)H-Index: 6
Last.Haile Ma (Jiangsu University)H-Index: 19
view all 8 authors...
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