Enzymatically modified starch with low digestibility produced from amylopectin by sequential amylosucrase and pullulanase treatments

Enzymatically modified starch with controllable digestibility may find unique applications. In the present study, side chains of amylopectin were elongated by amylosucrase, followed by debranching with pullulanase to produce linear chains that self-assembled to form microparticles containing aggregated nanoparticles. Amylosucrase treatment for a longer time resulted in longer branches that were not changed after the subsequent modification by...