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Structure and mechanisms of sodium-pumping KR2 rhodopsin.

Published on Apr 1, 2019in Science Advances
· DOI :10.1126/sciadv.aav2671
Kirill Kovalev5
Estimated H-index: 5
,
Vitaly Polovinkin7
Estimated H-index: 7
(CNRS: Centre national de la recherche scientifique)
+ 12 AuthorsValentin I. Gordeliy21
Estimated H-index: 21
(MIPT: Moscow Institute of Physics and Technology)
Abstract
Rhodopsins are the most universal biological light-energy transducers and abundant phototrophic mechanisms that evolved on Earth and have a remarkable diversity and potential for biotechnological applications. Recently, the first sodium-pumping rhodopsin KR2 from Krokinobacter eikastus was discovered and characterized. However, the existing structures of KR2 are contradictory, and the mechanism of Na + pumping is not yet understood. Here, we present a structure of the cationic (non H + ) light-driven pump at physiological pH in its pentameric form. We also present 13 atomic structures and functional data on the KR2 and its mutants, including potassium pumps, which show that oligomerization of the microbial rhodopsin is obligatory for its biological function. The studies reveal the structure of KR2 at nonphysiological low pH where it acts as a proton pump. The structure provides new insights into the mechanisms of microbial rhodopsins and opens the way to a rational design of novel cation pumps for optogenetics.
  • References (47)
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References47
Newest
#1Jagdeep Kaur (Goethe University Frankfurt)H-Index: 3
#2Clara Nassrin Kriebel (Goethe University Frankfurt)H-Index: 1
Last.Christian Bamann (MPG: Max Planck Society)H-Index: 20
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#1Christiane Grimm (Humboldt University of Berlin)H-Index: 5
#2Arita Silapetere (Humboldt University of Berlin)H-Index: 2
Last.Peter Hegemann (Humboldt University of Berlin)H-Index: 57
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#1Vitaly Shevchenko (MIPT: Moscow Institute of Physics and Technology)H-Index: 5
#2Thomas Mager (MPG: Max Planck Society)H-Index: 5
Last.Rohit GhaiH-Index: 29
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#1Daria N. Shalaeva (MSU: Moscow State University)H-Index: 4
#2Dmitry A. Cherepanov (MSU: Moscow State University)H-Index: 24
Last.Armen Y. Mulkidjanian (MSU: Moscow State University)H-Index: 31
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#1Takefumi Morizumi (U of T: University of Toronto)H-Index: 17
#2Wei-Lin Ou (U of T: University of Toronto)H-Index: 4
Last.Oliver P. Ernst (U of T: University of Toronto)H-Index: 32
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#1Kirill KovalevH-Index: 5
#2Dmytro Volkov (Forschungszentrum Jülich)
Last.Alexander Popov (European Synchrotron Radiation Facility)H-Index: 18
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View next paperThe light‐driven sodium ion pump: A new player in rhodopsin research