Perdeuteration, large crystal growth and neutron data collection ofLeishmania mexicanatriose-phosphate isomerase E65Q variant

Triose-phosphate isomerase (TIM) catalyses the interconversion of dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Two catalytic mechanisms have been proposed based on two reaction-intermediate analogues, 2-phosphoglycolate (2PG) and phosphoglycolohydroxamate (PGH), that have been used as mimics of the cis -enediol(ate) intermediate in several studies of TIM. The protonation states that are critical for the mechanistic interpretation...