Temperature-induced nucleation and growth of protein single crystals

Two techniques for the nucleation and growth of single crystals of biological macromolecules are proposed. The first one utilizes a very slow temperature shift at a capillary point where the crystal is to be grown. This allows to suppress an undesirable multiple nucleation. The second technique includes several local rapid temperature changes (a temperature “shock”) forcing the nucleation at the given point. These techniques were successfully...