Pretreatment and gaseous radiocarbon dating of 40–100 mg archaeological bone

Published on Dec 1, 2019in Scientific Reports4.011
· DOI :10.1038/s41598-019-41557-8
Helen Fewlass1
Estimated H-index: 1
(MPG: Max Planck Society),
Thibaut Tuna4
Estimated H-index: 4
(AMU: Aix-Marseille University)
+ 4 AuthorsSahra Talamo23
Estimated H-index: 23
(MPG: Max Planck Society)
Radiocarbon dating archaeological bone typically requires 300–1000 mg material using standard protocols. We report the results of reducing sample size at both the pretreatment and 14C measurement stages for eight archaeological bones spanning the radiocarbon timescale at different levels of preservation. We adapted our standard collagen extraction protocol specifically for <100 mg bone material. Collagen was extracted at least twice (from 37–100 mg material) from each bone. Collagen aliquots containing <100 μg carbon were measured in replicate using the gas ion source of the AixMICADAS. The effect of sample size reduction in the EA-GIS-AMS system was explored by measuring 14C of collagen containing either ca. 30 μg carbon or ca. 90 μg carbon. The gas dates were compared to standard-sized graphite dates extracted from large amounts (500–700 mg) of bone material pretreated with our standard protocol. The results reported here demonstrate that we are able to reproduce accurate radiocarbon dates from <100 mg archaeological bone material back to 40,000 BP.
  • References (58)
  • Citations (1)
📖 Papers frequently viewed together
3 Authors (Rachel Wood, ..., Thomas Higham)
45 Citations
11 Citations
4 Authors (F. Asgharizadeh, ..., O Houjaghani)
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Negar Haghipour (ETH Zurich)H-Index: 9
#2Blanca Ausín (ETH Zurich)H-Index: 3
Last. Timothy I. Eglinton (ETH Zurich)H-Index: 61
view all 8 authors...
We examine instrumental and methodological capabilities for microscale (10–50 μg of C) radiocarbon analysis of individual compounds in the context of paleoclimate and paleoceanography applications, for which relatively high-precision measurements are required. An extensive suite of data for 14C-free and modern reference materials processed using different methods and acquired using an elemental-analyzer–accelerator-mass-spectrometry (EA-AMS) instrumental setup at ETH Zurich was compiled to asses...
2 CitationsSource
#1Thibaut Tuna (AMU: Aix-Marseille University)H-Index: 4
#2Yoann Fagault (AMU: Aix-Marseille University)H-Index: 4
Last. Edouard Bard (AMU: Aix-Marseille University)H-Index: 73
view all 5 authors...
Abstract After three years of tests and developments, the performances of the AixMICADAS facility have been established for small CO2 samples with a carbon mass inferior to 100 μg. The instrument shows very good stability and reliability when measuring small samples with its gas ion source. In this configuration, the precision is mainly limited by counting statistics and also the accuracy by contamination effects for the smallest samples (
8 CitationsSource
#1Julia GottschalkH-Index: 8
#2Sönke SzidatH-Index: 40
Last. Samuel L. JaccardH-Index: 28
view all 8 authors...
Radiocarbon (14C) measurements of foraminifera often provide the only absolute age constraints in marine sediments. However, they are often challenging as their reliability and accuracy can be compromised by reduced availability of adequate sample material. New analytical advances using the MIni CArbon DAting System (MICADAS) allow 14C dating of very small samples, circumventing size limitations inherent to conventional 14C measurements with accelerator mass spectrometry (AMS). Here we use foram...
5 CitationsSource
#1Petra Korlević (MPG: Max Planck Society)H-Index: 6
#2Sahra Talamo (MPG: Max Planck Society)H-Index: 23
Last. Matthias Meyer (MPG: Max Planck Society)H-Index: 51
view all 3 authors...
Current protocols for ancient DNA and radiocarbon analysis of ancient bones and teeth call for multiple destructive samplings of a given specimen, thereby increasing the extent of undesirable damage to precious archaeological material. Here we present a method that makes it possible to obtain both ancient DNA sequences and radiocarbon dates from the same sample material. This is achieved by releasing DNA from the bone matrix through incubation with either EDTA or phosphate buffer prior to comple...
6 CitationsSource
#1Sophie Cersoy (University of Paris)H-Index: 2
#2Antoine Zazzo (University of Paris)H-Index: 20
Last. Nadine Tisnérat-Laborde (Université Paris-Saclay)H-Index: 19
view all 9 authors...
Because hard tissues can be radiocarbon dated, they are key to establishing the archaeological chronologies, palaeoenvironmental reconstructions and historical-biogeographical processes of the last 50,000 years. The advent of accelerator mass spectrometers (AMS) has revolutionized the field of archaeology but routine AMS dating still requires 60–200 mg of bone, which far exceeds that of small vertebrates or remains which hold a patrimonial value (e.g. hominid remains or worked bone artefacts). H...
9 CitationsSource
#1Helen Fewlass (MPG: Max Planck Society)H-Index: 1
#2Sahra Talamo (MPG: Max Planck Society)H-Index: 23
Last. Edouard BardH-Index: 73
view all 9 authors...
Abstract For many of archaeology’s rarest and most enigmatic bone artifacts (e.g. human remains, bone ornaments, worked bone), the destruction of the 500 mg material necessary for direct accelerator mass spectrometry (AMS) dating on graphite targets would cause irreparable damage; therefore many have not been directly dated. The recently improved gas ion source of the MICADAS (MIni CArbon DAting System) offers a solution to this problem by measuring gaseous samples of 5–100 µg carbon at a level ...
6 CitationsSource
#1Peter SteierH-Index: 32
#2Jakob LieblH-Index: 4
Last. Robin GolserH-Index: 20
view all 5 authors...
3 CitationsSource
#1Lise Bonvalot (AMU: Aix-Marseille University)H-Index: 3
#2Thibaut Tuna (AMU: Aix-Marseille University)H-Index: 4
Last. Edouard Bard (AMU: Aix-Marseille University)H-Index: 73
view all 7 authors...
Atmospheric particulate matter (PM) affects the climate in various ways and has a negative impact on human health. In populated mountain valleys in Alpine regions , emissions from road traffic contribute to carbonaceous aerosols, but residential wood burning can be another source of PM during winter. We determine the contribution of fossil and non-fossil carbon sources by measuring radiocarbon in aerosols using the recently installed AixMICADAS facility. The accelerator mass spectrometer is coup...
20 CitationsSource
#1S CersoyH-Index: 1
#2Antoine ZazzoH-Index: 20
Last. Séverine ZirahH-Index: 17
view all 5 authors...
11 CitationsSource
#1Cameron McIntyreH-Index: 17
#2Lukas WackerH-Index: 42
Last. Hans-Arno SynalH-Index: 26
view all 8 authors...
Studies using carbon isotopes to understand the global carbon cycle are critical to identify and quantify sources, sinks, and processes and how humans may impact them. 13C and 14C are routinely measured individually; however, there is a need to develop instrumentation that can perform concurrent online analyses that can generate rich data sets conveniently and efficiently. To satisfy these requirements, we coupled an elemental analyzer to a stable isotope mass spectrometer and an accelerator mas...
15 CitationsSource
Cited By1
#1Helen Fewlass (MPG: Max Planck Society)H-Index: 1
#2Sahra Talamo (UNIBO: University of Bologna)H-Index: 23
Last. Jiri Svoboda (Masaryk University)H-Index: 34
view all 12 authors...
Abstract The ritual human burials and scattered fragments of human bones excavated from Dolni Věstonice II and Pavlov I (Czech Republic) in the 20th century provide a large body of evidence on morphology and funerary practices in the Gravettian as well as the population history of European Homo sapiens during the Upper Palaeolithic. A series of radiocarbon dates on charcoal and animal bone places the occupation of the sites predominantly between 31,000–29,000 cal BP (Early-Evolved Pavlovian) but...
1 CitationsSource