Original paper
pH-Lemon, a Fluorescent Protein-Based pH Reporter for Acidic Compartments
Abstract
Distinct subcellular pH levels, especially in lysosomes and endosomes, are essential for the degradation, modification, sorting, accumulation, and secretion of macromolecules. Here, we engineered a novel genetically encoded pH probe by fusing the pH-stable cyan fluorescent protein (FP) variant, mTurquoise2, to the highly pH-sensitive enhanced yellow fluorescent protein, EYFP. This approach yielded a ratiometric biosensor-referred to as...
Paper Details
Title
pH-Lemon, a Fluorescent Protein-Based pH Reporter for Acidic Compartments
Published Date
Mar 13, 2019
Journal
Volume
4
Issue
4
Pages
883 - 891
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Notes
History