Cellular and nuclear hepatocyte ploidy represent a repository in regenerating livers

The adult liver consists of various ploidy classes of hepatocytes, including mononuclear and binuclear cells. Nuclear division without subsequent cytokinesis is generating the different ploidy classes of hepatocytes postnatally. The exact fraction of each hepatocyte class in livers that undergo resection is still not exactly described. We developed 3D analysis protocols based on vibratome liver slices (100 µm thick) for such detailed...