Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds
Abstract
CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage still requires improvements. Here, we harness six novel Cas12a orthologs for genome editing in human and mouse cells, some of which utilize simple protospacer adjacent motifs (PAMs) that...
Paper Details
Title
Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds
Published Date
Feb 5, 2019
Journal
Volume
20
Issue
1
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