Using an Oligonucleotide-based Enzyme Capture Assay and Physiological Activation by the Thrombin-Thrombomodulin Complex Improves Protein C Deficiency Testing

Objectives: Hereditary Protein C (PC) deficiency is associated with a higher thrombotic risk. Chromogenic PC activity tests, utilizing Protac, a PC activator isolated from Agkistrodon contortrix venom, are commonly used for diagnosing hereditary PC deficiency. The Protac-based mode of PC activation differs from endothelial cell-dependent activation through the thrombin-thrombomodulin (TTM) complex. Therefore, PC activity measured by Protac-based...