Surveillance on antibodies against West Nile virus, Usutu virus, tick-borne encephalitis virus and Tribeč virus in wild birds in Drienovská wetland, Slovakia

Published on Jul 1, 2019in Biologia0.728
· DOI :10.2478/s11756-019-00211-4
Tomáš Csank5
Estimated H-index: 5
Ľuboš Korytár1
Estimated H-index: 1
+ 3 AuthorsAlexander Csanády1
Estimated H-index: 1
(University of Prešov)
Searching for specific antibodies in bird serum could reveal arbovirus activity in certain localities. To date, no systematic surveillance for arbovirus infection in autochthonous population of wild birds has been conducted in Slovakia. Present study includes birds captured on spring migration and birds of the autochthonous nesting population of the Drienovska wetland (Slovakia). Serum samples were tested for West Nile virus (WNV), Usutu virus (USUV), Tick-borne encephalitis virus (TBEV) and Tribec virus (TRBV) neutralizing antibodies (NAb). Blood samples were collected during 2017 spring migration (March – April) and from local breeders and hatched juveniles (May – July) caught by Constant Effort site ringing method. Specific NAb were detected by micro-titration virus neutralization test. WNV NAb were detected in 11.7% (18/153) of sampled birds. Ten WNV seropositive were migrants (Eurasian Robins, Eurasian Blackcap and a Great Tit), 5 were nesting adults (Song Thrush, White Throat, Eurasian Jay, Hawfinch and Eurasian Blackbird) and 3 were juveniles (Song Thrush, Common Starling and a Great Tit). There was not confirmed USUV infection in any of the examined birds. TBEV NAb were detected during breeding season in the adult Eurasian Blackcap, which means 1.1% (1/93) seropositivity. TRBV NAb was detected in 7.4% (5/68) of adult birds (Eurasian Blackbirds and a Common Starling) and in one juvenile Great Tit, which had also WNV Nab as well. These results suggest active transmission of at least two arboviruses in the autochthonous avifauna in Slovakia.
  • References (47)
  • Citations (1)
📖 Papers frequently viewed together
20 Citations
3 Citations
3 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1CsankTomášH-Index: 1
#2DrzewniokováPetraH-Index: 1
Last. BakonyiTamás (University of Veterinary Medicine Vienna)H-Index: 1
view all 7 authors...
3 CitationsSource
The arbovirus surveillance and research programme was funded by several grants over the years including the Poliomyelitis Research Foundation of South Africa, the National Research Foundation, the Medical Research council and the Global Disease Detection Programme of the US-CDC (1 U19GH00057102: Investigation of vector-borne viruses as the cause of neurological disease of humans and animals.) Collaboration agreement with the National Institute for Communicable Diseases).
5 CitationsSource
#1Tamás BakonyiH-Index: 31
#2Károly ErdélyiH-Index: 20
Last. Norbert Nowotny (University of Medicine and Health Sciences)H-Index: 46
view all 6 authors...
Usutu virus (USUV, Flaviviridae) was first reported in Europe in Austria in 2001, where it caused wild bird (mainly blackbird) mortality until 2005. Since 2006 no further USUV cases were diagnosed ...
17 CitationsSource
#1Z. MojžišováH-Index: 1
Last. Juraj PistlH-Index: 10
view all 8 authors...
1 CitationsSource
#1Giuseppe ManciniH-Index: 3
#2Fabrizio MontarsiH-Index: 21
Last. Giovanni SaviniH-Index: 28
view all 14 authors...
22 CitationsSource
#1Tomáš CsankH-Index: 5
#2Katarina BhideH-Index: 4
Last. Juraj PistlH-Index: 10
view all 10 authors...
West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by sequencing. WNV was detected in swab and brain samples, whereas one brain sample was positive for tick-borne encephalitis virus (TBEV). The WNV sequenc...
9 CitationsSource
#1Lenka Berthová (SAV: Slovak Academy of Sciences)H-Index: 8
#2Slobodník (TNC: The Nature Conservancy)H-Index: 1
Last. Eva Špitalská (SAV: Slovak Academy of Sciences)H-Index: 16
view all 8 authors...
Ixodid ticks (Acari: Ixodidae) are known as primary vectors of many pathogens causing diseases in humans and animals. Ixodes ricinus is a common ectoparasite in Europe and birds are often hosts of subadult stages of the tick. From 2012 to 2013, 347 birds belonging to 43 species were caught and examined for ticks in three sites of Slovakia. Ticks and blood samples from birds were analysed individually for the presence of Rickettsia spp. and Coxiella burnetii by PCR-based methods. Only I. ricinus ...
18 CitationsSource
#1Eileen M. McKee (Governors State University)H-Index: 1
#2Edward D. Walker (MSU: Michigan State University)H-Index: 16
Last. Gabriel L. Hamer (A&M: Texas A&M University)H-Index: 21
view all 13 authors...
Antibody duration, following a humoral immune response to West Nile virus (WNV) infection, is poorly understood in free-ranging avian hosts. Quantifying antibody decay rate is important for interpreting serologic results and for understanding the potential for birds to serorevert and become susceptible again. We sampled free-ranging birds in Chicago, Illinois, US, from 2005 to 2011 and Atlanta, Georgia, US, from 2010 to 2012 to examine the dynamics of antibody decay following natural WNV infecti...
6 CitationsSource
#1Usama AshrafH-Index: 8
#2Jing YeH-Index: 14
Last. Shengbo Cao (HAU: Huazhong Agricultural University)H-Index: 19
view all 6 authors...
Usutu virus (USUV) is an African mosquito-borne flavivirus belonging to the Japanese encephalitis virus serocomplex. USUV is closely related to Murray Valley encephalitis virus, Japanese encephalitis virus, and West Nile virus. USUV was discovered in South Africa in 1959. In Europe, the first true demonstration of circulation of USUV was reported in Austria in 2001 with a significant die-off of Eurasian blackbirds. In the subsequent years, USUV expanded to neighboring countries, including Italy,...
70 CitationsSource
#1Daniel CadarH-Index: 19
#2Norbert BeckerH-Index: 25
Last. Jonas Schmidt-ChanasitH-Index: 38
view all 6 authors...
To the Editor: Usutu virus (USUV) is an arthropod-borne flavivirus that belongs to the Japanese encephalitis serocomplex (1). USUV circulates between ornithophilic mosquito vectors (mainly Culex spp. mosquitoes) and avian amplification hosts (2). Migratory birds play a key role in the introduction of USUV into new areas (3). USUV has recently been introduced from Africa into Europe, causing epizootics among wild birds and Usutu fever in humans (4–6). The detection and isolation of USUV from diff...
34 CitationsSource
Cited By1
#2Silvie ŠikutováH-Index: 11
Last. Ivo RudolfH-Index: 18
view all 11 authors...
Monitoring West Nile virus (WNV) and Usutu virus (USUV) activity now has the highest priority among mosquito-borne pathogenic viruses circulating in the European Union. This study documents a first time detection and the co-circulation of WNV lineage-2 (with the minimal prevalence of 0.46%) and USUV clade Europe 2 (with the minimal prevalence of 0.25%) in mosquitoes from the same habitat of south-western Slovakia and underlines necessity to perform rigorous surveillance in birds, mosquitoes, hor...