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Assessment of food microbiological indicators applied on poultry carcasses by culture combined MALDI-TOF MS identification and 16S rRNA amplicon sequencing

Published on Sep 1, 2019in Food Microbiology 4.09
· DOI :10.1016/j.fm.2019.01.018
Zhongjia Yu (UGent: Ghent University), Maria Francesca Peruzy2
Estimated H-index: 2
(UGent: Ghent University)
+ 2 AuthorsKurt Houf33
Estimated H-index: 33
(UGent: Ghent University)
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Abstract
Abstract Examination of the bacterial contamination on food products is still largely performed by standardized culture methods, though culture-independent methods are suggested as a more reliable approach. Knowledge of the diversity of bacteria isolated from food as well as the impact of the plate incubation conditions applied are still understudied. The impact of incubation at 7 °C and 30 °C on total aerobic bacterial count and diversity, and the performance of ISO methods generally applied in microbiological quality examination were assessed by culture combined MALDI-TOF MS identification and 16S rRNA amplicon sequencing. Examining breast skin of 16 chicken carcasses, no significant impact of the incubation temperature on the total aerobic bacteria level and diversity was detected, limiting the usefulness of additional psychrophilic examination. Bacteria phenotypically similar to Pseudomonas, were identified on selective CFC plates, and on MRS agar plates for lactic acid bacteria, Escherichia coli and Staphylococcus were commonly present. Application of 16S rRNA amplicon sequencing revealed a higher bacterial diversity, but the impact of the DNA extraction kit applied, and the detection of non-viable bacteria should be taken into account to interpret the final outcome.
  • References (32)
  • Citations (1)
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References32
Newest
Published on Apr 1, 2018in Food Microbiology 4.09
Amélie Rouger3
Estimated H-index: 3
(INRA: Institut national de la recherche agronomique),
Nicolas Moriceau1
Estimated H-index: 1
(INRA: Institut national de la recherche agronomique)
+ 2 AuthorsMonique Zagorec33
Estimated H-index: 33
(INRA: Institut national de la recherche agronomique)
Abstract Poultry meat, the second most consumed meat in France, is commercialized mainly as portions of chicken cuts with various quality labels, stored under various modified atmosphere packaging (MAP), with shelf-life ranging from 9 to 17 days. We used 16S rDNA pyrosequencing to describe microbiota of chicken legs. Ten samples representing a wide diversity of labels and MAP available on the market were collected from local supermarkets and stored at 4 °C. Microbiota were collected, total DNA w...
Published on Oct 2, 2017in Nature Biotechnology 31.86
Paul Igor Costea15
Estimated H-index: 15
,
Georg Zeller29
Estimated H-index: 29
+ 54 AuthorsFerris Elias Jung1
Estimated H-index: 1
Technical variation in metagenomic analysis must be minimized to confidently assess the contributions of microbiota to human health. Here we tested 21 representative DNA extraction protocols on the same fecal samples and quantified differences in observed microbial community composition. We compared them with differences due to library preparation and sample storage, which we contrasted with observed biological variation within the same specimen or within an individual over time. We found that D...
Published on Apr 1, 2017in International Journal of Food Microbiology 4.01
Emilie Cauchie1
Estimated H-index: 1
(University of Liège),
Mathieu Gand1
Estimated H-index: 1
(University of Liège)
+ 4 AuthorsGeorges Daube31
Estimated H-index: 31
(University of Liège)
Abstract In order to control food losses and wastage, monitoring the microbial diversity of food products, during processing and storage is important, as studies have highlighted the metabolic activities of some microorganisms which can lead to spoilage. Knowledge of this diversity can be greatly improved by using a metagenetic approach based on high throughput 16S rRNA gene sequencing, which enables a much higher resolution than culture-based methods. Moreover, the Jameson effect, a phenomenon ...
Published on Apr 1, 2017in International Journal of Food Microbiology 4.01
Amélie Rouger3
Estimated H-index: 3
(INRA: Institut national de la recherche agronomique),
Benoit Remenant7
Estimated H-index: 7
(INRA: Institut national de la recherche agronomique)
+ 1 AuthorsMonique Zagorec33
Estimated H-index: 33
(INRA: Institut national de la recherche agronomique)
Abstract Influenced by production and storage processes and by seasonal changes the diversity of meat products microbiota can be very variable. Because microbiotas influence meat quality and safety, characterizing and understanding their dynamics during processing and storage is important for proposing innovative and efficient storage conditions. Challenge tests are usually performed using meat from the same batch, inoculated at high levels with one or few strains. Such experiments do not reflec...
Published on Dec 1, 2016in Food Microbiology 4.09
Linda Höll2
Estimated H-index: 2
(TUM: Technische Universität München),
Jürgen Behr15
Estimated H-index: 15
(TUM: Technische Universität München),
Rudi F. Vogel55
Estimated H-index: 55
(TUM: Technische Universität München)
Abstract Modified atmosphere packaging (MAP) is widely used in food industry to extend the microbiological shelf-life of meat. Typically, poultry meat has been packaged in a CO 2 /N 2 atmosphere (with residual low O 2 ). Recently, some producers use high O 2 MAP for poultry meat to empirically reach comparable shelf lifes. In this work, we compared spoilage microbiota of skinless chicken breast in high (80% O 2 , 20% CO 2 ) and low O 2 MAP (65% N 2 and 35% CO 2 ). Two batches of meat were incuba...
Published on Jul 1, 2016in Meat Science 3.48
Evelyne Mann9
Estimated H-index: 9
,
Stefanie U. Wetzels7
Estimated H-index: 7
+ 3 AuthorsStephan Schmitz-Esser24
Estimated H-index: 24
Abstract The aim of this study was to disentangle the microbial diversity on porcine musculature. The hypervariable V1–V2 region of the 16S rRNA gene was amplified from DNA samples of clinically healthy slaughter pigs (n = 8). Pyrosequencing yielded 37,000 quality-controlled reads and a diverse microbiome with 54–159 OTUs per sample was detected. Interestingly, 6 out of 8 samples were strongly dominated by 1–2 highly abundant OTUs (best hits of highly abundant OTUs: Serratia proteamaculans , Pse...
Published on Apr 15, 2016in Applied and Environmental Microbiology 4.08
Xiang Yang6
Estimated H-index: 6
(CSU: Colorado State University),
Noelle R. Noyes7
Estimated H-index: 7
(CSU: Colorado State University)
+ 11 AuthorsKenneth L. Jones23
Estimated H-index: 23
(University of Colorado Denver)
Foodborne illnesses associated with pathogenic bacteria are a global public health and economic challenge. The diversity of microorganisms (pathogenic and nonpathogenic) that exists within the food and meat industries complicates efforts to understand pathogen ecology. Further, little is known about the interaction of pathogens within the microbiome throughout the meat production chain. Here, a metagenomic approach and shotgun sequencing technology were used as tools to detect pathogenic bacteri...
Published on Jan 1, 2016in Korean Journal of Laboratory Medicine
Seri Jeong7
Estimated H-index: 7
(Kosin University),
Jun Sung Hong4
Estimated H-index: 4
(Yonsei University)
+ 5 AuthorsSeok Hoon Jeong34
Estimated H-index: 34
(Yonsei University)
Acinetobacter baumannii has a greater clinical impact and exhibits higher antimicrobial resistance rates than the non-baumannii Acinetobacter species. Therefore, the correct identification of Acinetobacter species is clinically important. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has recently become the method of choice for identifying bacterial species. The purpose of this study was to evaluate the ability of MALDI-TOF MS (Bruker Daltonics Gmb...
Published on Jul 30, 2015in Marine Drugs 3.77
Charles R. Sweet2
Estimated H-index: 2
,
Rebecca E. Watson1
Estimated H-index: 1
(PSU: Pennsylvania State University)
+ 1 AuthorsJoseph P. Smith9
Estimated H-index: 9
(USNA: United States Naval Academy)
Lipid A is a fundamental Gram-negative outer membrane component and the essential element of lipopolysaccharide (endotoxin), a potent immunostimulatory molecule. This work describes the metabolic adaptation of the lipid A acyl structure by Psychrobacter cryohalolentis at various temperatures in its facultative psychrophilic growth range, as characterized by MALDI-TOF MS and FAME GC-MS. It also presents the first elucidation of lipid A structure from the Colwellia genus, describing lipid A from s...
Published on Dec 1, 2014in Food Microbiology 4.09
H. Meredith4
Estimated H-index: 4
(Ulster University),
V.P. Valdramidis23
Estimated H-index: 23
(University of Malta)
+ 3 AuthorsDeclan Bolton29
Estimated H-index: 29
(Teagasc)
Studies were undertaken to investigate the effect of different modified atmospheric packaging (MAP) gaseous combinations on Campylobacter and the natural microflora on poultry fillets. Skinless chicken fillets were stored in gaseous mixtures of 10%, 30%, 50%, 70% and 90% CO2 balanced with N2, 80:20% O2:N2 and 40:30:30% CO2:O2:N2 and control conditions (air) at 2 � C. Samples were analysed periodically for (previously inoculated) Campylobacter, total viable counts (TVC) (mesophiles), TVC (psychro...
Cited By1
Newest
Published on Jul 1, 2019in International Journal of Food Microbiology 4.01
Maria Francesca Peruzy2
Estimated H-index: 2
,
Nicoletta Murru6
Estimated H-index: 6
+ 5 AuthorsKurt Houf33
Estimated H-index: 33
Abstract Wild boars ( Sus scrofa ) are the most widely distributed large mammals and recent increase in consumption of wild boar meat urges the need of microbiological quality criteria. The aim of the study was to characterize the initial bacterial contamination on freshly-killed wild boar meat using a culture-dependent approach with ISO-methods combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification and 16S rRNA amplicon sequencing. Moreover, th...