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Traceability of different brands of bottled mineral water during shelf life, using PCR-DGGE and next generation sequencing techniques

Published on Sep 1, 2019in Food Microbiology 4.09
· DOI :10.1016/
Laura Sala-Comorera3
Estimated H-index: 3
(University of Barcelona),
Anicet R. Blanch36
Estimated H-index: 36
(University of Barcelona)
+ 2 AuthorsCristina García-Aljaro17
Estimated H-index: 17
(University of Barcelona)
Abstract Natural mineral waters contain indigenous bacteria characteristic of each spring source. Once bottled, these communities change over time until the water is consumed. Bottle material is believed to play a major role in the succession of these populations, but very few studies to date have evaluated the effect of this material on bacterial communities. In this study, we examined the microbial community structure of three natural mineral waters over 3 months after bottling in glass and polyethylene terephthalate (PET) bottles. To this end, we used culture-dependent (heterotrophic plate count) and culture-independent methods (16S rRNA massive gene sequencing, denaturing gradient gel electrophoresis (DGGE) and fluorescent microscopy with vital dyes). Total and viable cell counts increased by around 1–2 log 10 units between 1 and 2 weeks after bottling and then remained constant over 3 months for all waters regardless of the bottle material. DGGE fingerprints and 16S rRNA massive sequencing analysis both indicated that different communities were established in the waters two weeks after bottling in the different bottle materials. In conclusion, no differences in total, viable and culturable bacteria counts were observed between mineral waters bottled with PET or glass during shelf life storage. Nevertheless, in spite of changes in the communities, each water brand and material presented a distinct microbial community structure clearly distinguishable from the others, which could be interesting for traceability purposes.
  • References (33)
  • Citations (0)
Gerard Muyzer70
Estimated H-index: 70
(Max Planck Society),
Kornelia Smalla1
Estimated H-index: 1
Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so important.
1,791 Citations Source Cite
Published on May 23, 2009in Water SA 0.78
M.M. Burtscher6
Estimated H-index: 6
(Vienna University of Technology),
Franziska Zibuschka8
Estimated H-index: 8
(University of Agricultural Sciences, Dharwad)
+ 2 AuthorsAndreas H. Farnleitner27
Estimated H-index: 27
(Vienna University of Technology)
Heterotrophic plate count using ISO 6222 agar (HPC) vs. in situ bacterial (DF) community structure from corresponding samples of a drinking water distribution system were investigated by 16S rRNA gene-based polymerase chain reaction denaturing gradient gel electrophoresis (PCR DGGE) profiling. The investigation regime covered 10 different sampling locations and 2 points in time (t1, t2). In order to ensure accurate and reproducible 16S rRNA gene profile analysis, rigorous methodical evaluation a...
25 Citations Source Cite
Published on Jan 1, 2015in Journal of Microbiological Methods 1.70
A.D. Samarajeewa1
Estimated H-index: 1
(Environment Canada),
A. Hammad1
Estimated H-index: 1
(Environment Canada)
+ 3 AuthorsLee A. Beaudette7
Estimated H-index: 7
(Environment Canada)
Abstract Characterization of commercial microbial consortia products for human and environmental health risk assessment is a major challenge for regulatory agencies. As a means to develop an approach to assess the potential environmental risk of these products, research was conducted to compare four genomics methods for characterizing bacterial communities; (i) Denaturing Gradient Gel Electrophoresis (DGGE), (ii) Clonal-Restriction Fragment Length Polymorphism (C/RFLP), (iii) partial 16S rDNA am...
19 Citations Source Cite
Published on Jun 1, 1999in Journal of Applied Microbiology 2.16
C. Defives2
Estimated H-index: 2
S. Guyard2
Estimated H-index: 2
+ 2 AuthorsJ. P. Hornez2
Estimated H-index: 2
The changes in bacterial counts during the storage of a natural mineral water from a French spring were studied. Samples were taken from the spring and the bottling line. Viable cultivable (VC) bacteria were counted on R2A medium. Total counts, viable and dead bacteria were counted using the LIVE/DEAD® Bac LightTM VIABILITY kit and epifluorescence microscopy. Viable but non-cultivable (VNC) bacteria were estimated by difference between viable and VC counts. Isolates were clustered by phenotype. ...
43 Citations Source Cite
Ivone Vaz-Moreira18
Estimated H-index: 18
(University of Porto),
Conceição Egas16
Estimated H-index: 16
(University of Coimbra)
+ 1 AuthorsCélia M. Manaia35
Estimated H-index: 35
Compared with culture-independent approaches, traditionally used culture-dependent methods have a limited capacity to characterize water microbiota. Nevertheless, for almost a century the latter have been optimized to detect and quantify relevant bacteria. A pertinent question is if culture-independent diversity surveys give merely an extended perspective of the bacterial diversity or if, even with a higher coverage, focus on a different set of organisms. We compared the diversity and phylogeny ...
72 Citations Source Cite
Published on Aug 1, 1990in International Journal of Food Microbiology 3.45
Bischofberger T1
Estimated H-index: 1
(ETH Zurich),
Cha Sk1
Estimated H-index: 1
(ETH Zurich)
+ 2 AuthorsSchmidt-Lorenz W1
Estimated H-index: 1
(ETH Zurich)
Abstract Quantitative and qualitative determinations of the bacterial flora of non-carbonated natural mineral water at the most important steps during bottling at a large water source yielded the following results: (i) Colony counts (on 1 :10 diluted plate count agar, incubated at 20°C for 14 days) for water of the five springs and the mixed water were less than 1 to 4 cfu ml −1 . The Gram-negative bacterial flora ( n = 50 isolates) showed a very different but constant spring specific species di...
63 Citations Source Cite
Published on Jul 1, 2012in Water Research 7.05
Maria Fernanda Falcone-Dias2
Estimated H-index: 2
(Sao Paulo State University),
Ivone Vaz-Moreira18
Estimated H-index: 18
Célia M. Manaia35
Estimated H-index: 35
Abstract The antibiotic resistance phenotypes of the cultivable bacteria present in nine batches of two Portuguese and one French brands of commercially available mineral waters were examined. Most of the 238 isolates recovered on R2A, Pseudomonas Isolation agar or on these culture media supplemented with amoxicillin or ciprofloxacin, were identified (based on 16S rRNA gene sequence analysis) as Proteobacteria of the divisions Beta , Gamma and Alpha . Bacteria resistant to more than three distin...
49 Citations Source Cite
Published on May 1, 2015in Scientific Reports 4.12
Rosine E. Tiodjio4
Estimated H-index: 4
Akihiro Sakatoku6
Estimated H-index: 6
+ 9 AuthorsMinoru Kusakabe34
Estimated H-index: 34
The aim of this study was to assess the microbial diversity associated with Lake Nyos, a lake with an unusual chemistry in Cameroon. Water samples were collected during the dry season on March 2013. Bacterial and archaeal communities were profiled using Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) approach of the 16S rRNA gene. The results indicate a stratification of both communities along the water column. Altogether, the physico-chemical data and microbial sequ...
17 Citations Source Cite
Published on Mar 1, 2006in International Journal of Food Microbiology 3.45
D. Venieri6
Estimated H-index: 6
(University of Patras),
Apostolos Vantarakis22
Estimated H-index: 22
(Democritus University of Thrace)
+ 1 AuthorsM. Papapetropoulou12
Estimated H-index: 12
(University of Patras)
The microbiological quality of 1527 samples of bottled noncarbonated (Fstill_) mineral water, purchased from retail outlets and derived from 10 manufacturing companies in Greece, was investigated during the period 1995–2003. Applying the membrane filter technique, the aliquots of water samples (250 ml) were analyzed for the presence and enumeration of total coliforms, Escherichia coli, Enterococcus spp. and Pseudomonas aeruginosa. Also, aerobic bacteria were counted as Heterotrophic Plate Count ...
42 Citations Source Cite
Published on Oct 1, 1992in Journal of Applied Microbiology 2.16
Athena Mavridou1
Estimated H-index: 1
Natural mineral water from a UK spring was monitored at various stages after it was pumped from the ground, through to bottling and during shelf life before consumption. Samples were collected in commercial PVC bottles, in PVC bottles previously sterilized and hand-filled and in glass bottles. The bacterial flora was counted on plate count agar (PCA) and on PCA diluted 10 times (PCA/10). The predominant bacteria were identified to genus level. Growth rates and nutrient types of isolates were det...
49 Citations Source Cite
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