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Traceability of different brands of bottled mineral water during shelf life, using PCR-DGGE and next generation sequencing techniques

Published on Sep 1, 2019in Food Microbiology 4.09
· DOI :10.1016/
Laura Sala-Comorera3
Estimated H-index: 3
(University of Barcelona),
Anicet R. Blanch36
Estimated H-index: 36
(University of Barcelona)
+ 2 AuthorsCristina García-Aljaro18
Estimated H-index: 18
(University of Barcelona)
Abstract Natural mineral waters contain indigenous bacteria characteristic of each spring source. Once bottled, these communities change over time until the water is consumed. Bottle material is believed to play a major role in the succession of these populations, but very few studies to date have evaluated the effect of this material on bacterial communities. In this study, we examined the microbial community structure of three natural mineral waters over 3 months after bottling in glass and polyethylene terephthalate (PET) bottles. To this end, we used culture-dependent (heterotrophic plate count) and culture-independent methods (16S rRNA massive gene sequencing, denaturing gradient gel electrophoresis (DGGE) and fluorescent microscopy with vital dyes). Total and viable cell counts increased by around 1–2 log 10 units between 1 and 2 weeks after bottling and then remained constant over 3 months for all waters regardless of the bottle material. DGGE fingerprints and 16S rRNA massive sequencing analysis both indicated that different communities were established in the waters two weeks after bottling in the different bottle materials. In conclusion, no differences in total, viable and culturable bacteria counts were observed between mineral waters bottled with PET or glass during shelf life storage. Nevertheless, in spite of changes in the communities, each water brand and material presented a distinct microbial community structure clearly distinguishable from the others, which could be interesting for traceability purposes.
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Published in Mbio 6.75
Celine Lesaulnier1
Estimated H-index: 1
(University of Vienna),
Craig W. Herbold21
Estimated H-index: 21
(University of Vienna)
+ -3 AuthorsGabriel Singer22
Estimated H-index: 22
(Leibniz Association)
Background Non-carbonated natural mineral waters contain microorganisms that regularly grow after bottling despite low concentrations of dissolved organic matter (DOM). Yet, the compositions of bottled water microbiota and organic substrates that fuel microbial activity, and how both change after bottling, are still largely unknown.
Published on Sep 1, 2016in Extremophiles 2.05
Lucia Kraková13
Estimated H-index: 13
(SAV: Slovak Academy of Sciences),
Katarína Šoltys6
Estimated H-index: 6
(Comenius University in Bratislava)
+ 4 AuthorsTomas Szemes11
Estimated H-index: 11
(Comenius University in Bratislava)
Different protocols based on Illumina high-throughput DNA sequencing and denaturing gradient gel electrophoresis (DGGE)-cloning were developed and applied for investigating hot spring related samples. The study was focused on three target genes: archaeal and bacterial 16S rRNA and mcrA of methanogenic microflora. Shorter read lengths of the currently most popular technology of sequencing by Illumina do not allow analysis of the complete 16S rRNA region, or of longer gene fragments, as was the ca...
Published on Mar 1, 2016in Food Control 4.25
Malwina Diduch3
Estimated H-index: 3
(GUT: Gdańsk University of Technology),
Żaneta Polkowska17
Estimated H-index: 17
(GUT: Gdańsk University of Technology),
Jacek Namiesnik55
Estimated H-index: 55
(GUT: Gdańsk University of Technology)
Abstract The ever-growing popularity of bottled water is the result of many factors. One of the most relevant is the widespread belief that bottled water is safer for drinking than tap water. In fact many literature sources describe the rapid growth of the amounts of microorganisms in bottled water samples following the bottling process. The main purpose of this article is to collect the information on the factors which may affect this phenomenon and to assess the suitability of the existing mic...
Published on May 1, 2015in Scientific Reports 4.01
Rosine E. Tiodjio4
Estimated H-index: 4
Akihiro Sakatoku6
Estimated H-index: 6
+ 9 AuthorsMinoru Kusakabe34
Estimated H-index: 34
The aim of this study was to assess the microbial diversity associated with Lake Nyos, a lake with an unusual chemistry in Cameroon. Water samples were collected during the dry season on March 2013. Bacterial and archaeal communities were profiled using Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) approach of the 16S rRNA gene. The results indicate a stratification of both communities along the water column. Altogether, the physico-chemical data and microbial sequ...
Published on Mar 1, 2015in Environmental Microbiology 5.15
Luís França7
Estimated H-index: 7
(UC: University of Coimbra),
Arantxa López-López20
Estimated H-index: 20
(CSIC: Spanish National Research Council)
+ 1 AuthorsMilton S. da Costa36
Estimated H-index: 36
(UC: University of Coimbra)
The research was funded by Fundacao para a Ciencia e a Tecnologia under the projects PEst-C/SAU/LA0001/2011 and PTDC/BIA-MIC/105247/2008. L. F. acknowledges a scholarship from FCT (SFRH/BD/40879/2007). R. R. M. and A. L. L. acknowledge the scientific support given by the Spanish Ministry of Economy with the projects CE-CSD2007-0005 and CGL2012–39627-C03-03, both supported as well with FEDER funds
Published on Jan 1, 2015in Journal of Microbiological Methods 1.80
A.D. Samarajeewa2
Estimated H-index: 2
(EC: Environment Canada),
A. Hammad1
Estimated H-index: 1
(EC: Environment Canada)
+ 3 AuthorsL.A. Beaudette7
Estimated H-index: 7
(EC: Environment Canada)
Characterization of commercial microbial consortia products for human and environmental health risk assessment is a major challenge for regulatory agencies. As a means to develop an approach to assess the potential environmental risk of these products, research was conducted to compare four genomics methods for characterizing bacterial communities; (i) Denaturing Gradient Gel Electrophoresis (DGGE), (ii) Clonal-Restriction Fragment Length Polymorphism (C/RFLP), (iii) partial 16S rDNA amplificati...
Published on Sep 1, 2014in Environmental Microbiology 5.15
Angélique Gobet15
Estimated H-index: 15
(MPG: Max Planck Society),
Antje Boetius66
Estimated H-index: 66
(Alfred Wegener Institute for Polar and Marine Research),
Alban Ramette37
Estimated H-index: 37
(MPG: Max Planck Society)
Changes in richness and bacterial community structure obtained via 454 Massively Parallel Tag Sequencing (MPTS) and Automated Ribosomal Intergenic Analysis (ARISA) were systematically compared to determine whether and how the ecological knowledge obtained from both molecular techniques could be combined. We evaluated community changes over time and depth in marine coastal sands at different levels of taxonomic resolutions, sequence corrections and sequence abundances. Although richness over dept...
Published on May 1, 2013in Food Control 4.25
Maria Fernanda Falcone-Dias2
Estimated H-index: 2
(UNESP: Sao Paulo State University),
Adalberto Farache Filho3
Estimated H-index: 3
(UNESP: Sao Paulo State University)
Abstract Quantitative variations in heterotrophic plate count (HPC) and in the presence of indicator microorganisms in 0.5, 1.5 and 20-L bottles of different brands of Brazilian mineral water were analyzed during their shelf life. No variations were identified in the presence of indicator microorganisms, but quantitative variations in HPC were observed in some brands, which suggests that changes may be occurring in the water quality during storage. The aim of this study was also to evaluate the ...
Published on Apr 22, 2013in PLOS ONE 2.78
Paul J. McMurdie16
Estimated H-index: 16
(Stanford University),
Susan Holmes44
Estimated H-index: 44
(Stanford University)
Background The analysis of microbial communities through DNA sequencing brings many challenges: the integration of different types of data with methods from ecology, genetics, phylogenetics, multivariate statistics, visualization and testing. With the increased breadth of experimental designs now being pursued, project-specific statistical analyses are often needed, and these analyses are often difficult (or impossible) for peer researchers to independently reproduce. The vast majority of the re...
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