Biopharmaceutical benchmarks—2003

Published on Aug 1, 2003in Nature Biotechnology
· DOI :10.1038/nbt0803-865
Gary Walsh23
Estimated H-index: 23
(UL: University of Limerick)
  • References (8)
  • Citations (130)
📖 Papers frequently viewed together
1,499 Citations
726 Citations
359 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
22 CitationsSource
52 CitationsSource
#1Kevin RobinsonH-Index: 1
s we approach the end of 2002, biotechnology companies continue to be the standard bearers for innovation in the pharmaceutical industry. And, according to a new publication from Scrip Reports (, even faster progress is expected following the initial sequencing of the complete human genome and the move to the new frontiers of proteomics (1). The U.S. biotech market is still the industry leader, with revenues of $22.4 billion in 2000. Yet, by comparison, the European biotech marke...
8 Citations
#1Alexander Pfeifer (Salk Institute for Biological Studies)H-Index: 53
#2Inder M. Verma I M (Salk Institute for Biological Studies)H-Index: 130
Gene therapy can be broadly defined as the transfer of genetic material to cure a disease or at least to improve the clinical status of a patient. One of the basic concepts of gene therapy is to transform viruses into genetic shuttles, which will deliver the gene of interest into the target cells. Based on the nature of the viral genome, these gene therapy vectors can be divided into RNA and DNA viral vectors. The majority of RNA virus-based vectors have been derived from simple retroviruses lik...
321 CitationsSource
#1Saghir Akhtar (Aston University)H-Index: 37
#2Marcus D. Hughes (University of Bradford)H-Index: 9
Last. Pakeeza Sayyed (University of Bradford)H-Index: 3
view all 8 authors...
Antisense oligonucleotides, ribozymes and DNAzymes have emerged as novel, highly selective inhibitors or modulators of gene expression. Indeed, their use in the treatment of diseases arising from genetic abnormalities has become a real possibility over the past few years. The first antisense drug molecule is now available for clinical use in Europe and USA. However, their successful application in the clinic will require improvements in cellular targeting and intracellular delivery. This review ...
203 CitationsSource
#1Dan Luo (Cornell University)H-Index: 38
#2W. M. Saltzman (Cornell University)H-Index: 3
The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology. Toward this end, rapid advances have recently been made in our understanding of mechanisms for DNA stability and transport within cells. Current synthetic DNA delivery systems are versatile and safe, but substantially less efficient than viruses. Indeed, most current systems address only one of the obstacles to DNA delivery by enhancing DNA uptake. In fact, the effectiveness of gene expr...
1,422 CitationsSource
26 Citations
#1Colin W. Pouton (University of Bath)H-Index: 44
#2L. W. Seymour (University of Birmingham)H-Index: 11
Abstract The future of non-viral gene therapy depends on a detailed understanding of the barriers to delivery of polynucleotides. These include physicomechanical barriers, which limit the design of delivery devices, physicochemical barriers that influence self-assembly of colloidal particulate formulations, and biological barriers that compromise delivery of the DNA to its target site. It is important that realistic delivery strategies are adopted for early clinical trials in non-viral gene ther...
374 CitationsSource
Cited By130
#1Jaber Dehghani (University of Tabriz)H-Index: 3
#2Yousef Javadzadeh (Tabriz University of Medical Sciences)H-Index: 28
Last. Yadollah Omidi (Tabriz University of Medical Sciences)H-Index: 33
view all 6 authors...
Abstract Most of the recent approved therapeutic proteins are multi-subunit biologics, which need glycosylation and disulfide bridges for the correct conformation and biological functions. Currently, there exist many protein-based drugs that are mostly produced in the Chinese hamster ovary (CHO) cells. However, this expression system appears to associate with some limitations both in upstream and downstream processing steps, including low growth rate, sensitivity to different stresses and pathog...
#1Hyo Helen ChungH-Index: 2
#2Lynette BuckH-Index: 3
Last. Jette WypychH-Index: 27
view all 6 authors...
: Monoclonal antibodies (mAbs) are composed of two heavy chain (HC) and two light chain (LC) polypeptides. The proper folding and assembly of HC and LC is critical for antibody production. Current dogma indicates that the free HCs are retained in the endoplasmic reticulum (ER) unless assembled with LCs into antibodies, while the LCs on the other hand can be secreted as free monomer or dimer molecules. In this study, high levels of extracellular HC homodimers (7%-45%) were observed in the cell cu...
2 CitationsSource
#1Lara E. Krebs (Eli Lilly and Company)H-Index: 2
#2Daniel M. Bowden (Eli Lilly and Company)H-Index: 1
Last. Christopher C. Frye (Eli Lilly and Company)H-Index: 6
view all 5 authors...
: Mammalian cell line development is critical to bioproduct manufacturing. Success requires selecting a line with desirable performance characteristics, including consistent expression throughout the proposed manufacturing window. Given the genetic and phenotypic flux inherent to immortalized lines such as Chinese hamster ovary cells, clonally-derived cell line characterization is vital. We describe here the development and implementation of a novel addition to our characterization approach to e...
1 CitationsSource
Abstract Yeast is very well known eukaryotic organism for its remarkable biodiversity and extensive industrial applications. Saccharomyces cerevisiae is one of the most widely used microorganisms in biotechnology with successful applications in the biochemical production. Biological conversion with the focus on the different utilization of renewable feedstocks into fuels and chemicals has been intensively investigated due to increasing concerns on sustainability issues worldwide. Compared with i...
7 CitationsSource
#1Fabian Steinebach (ETH Zurich)H-Index: 9
#2Daniel J. Karst (ETH Zurich)H-Index: 8
Last. Massimo Morbidelli (ETH Zurich)H-Index: 52
view all 3 authors...
Murine myeloma NS0 cells are cholesterol-dependent auxotrophs and require externally provided cholesterol for sustained growth. Traditionally, cholesterol is provided to these cells by supplementing cell culture media with a concentrated solution of cholesterol and other water insoluble components dissolved in 200-proof ethanol. However, the solubility of cholesterol in ethanol is limited, and for processes requiring large amounts of cholesterol, the consequential increase in added ethanol may n...
Typically, mammalian cell culture medium contains high glucose concentrations that are analogous to diabetic levels in humans, suggesting that mammalian cells are cultivated in excessive glucose. Using RNA-Seq, this study characterized the Chinese hamster ovary (CHO) cell transcriptome under two glucose concentrations to assess the genetic effects associated with metabolic pathways, in addition to other global responses. The initial extracellular glucose concentrations used represented high (30 ...
5 CitationsSource
#1Ling LiuH-Index: 3
Last. Jeanine L. BussiereH-Index: 13
view all 15 authors...
Abstract The stable effector functionLess (SEFL) antibody was designed as an IgG1 antibody with a constant region that lacks the ability to interact with Fcγ receptors. The engineering and stability and pharmacokinetic assessments of the SEFL scaffold is described in the accompanying article (Jacobsen, F. W., Stevenson, R., Li, C., Salimi-Moosavi, H., Liu, L., Wen, J., Luo, Q., Daris, K., Buck, L., Miller, S., Ho, S-Y., Wang, W., Chen, Q., Walker, K., Wypych, J., Narhi, L., and Gunasekaran, K. (...
3 CitationsSource
#1Judy Muller-CohnH-Index: 2
#2Paul DiazH-Index: 11
Last. Rolf MullerH-Index: 2
view all 3 authors...
2 CitationsSource
#1Lydia Lamriben (UMass: University of Massachusetts Amherst)H-Index: 3
#2Jill B. Graham (UMass: University of Massachusetts Amherst)H-Index: 2
Last. Daniel N. Hebert (UMass: University of Massachusetts Amherst)H-Index: 33
view all 4 authors...
Helenius and colleagues proposed over 20-years ago a paradigm-shifting model for how chaperone binding in the endoplasmic reticulum was mediated and controlled for a new type of molecular chaperone- the carbohydrate-binding chaperones, calnexin and calreticulin. While the originally established basics for this lectin chaperone binding cycle holds true today, there has been a number of important advances that have expanded our understanding of its mechanisms of action, role in protein homeostasis...
46 CitationsSource