Quantification of d-mannose in plasma: Development and validation of a reliable and accurate HPLC-MS-MS method

The present paper describes the development and the validation process - in compliance with the EMA guidelines - of a method based on tandem mass spectrometry coupled to liquid chromatography for the accurate quantification of mannose in human plasma samples. The quick sample preparation procedure, simplified by the absence of any derivatization step, makes the assay suitable for routine use in a clinical chemistry laboratory. The method...