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RNA-sequencing analysis of shell gland shows differences in gene expression profile at two time-points of eggshell formation in laying chickens

Published on Jan 25, 2019in BMC Genomics3.50
· DOI :10.1186/s12864-019-5460-4
Samiullah Khan1
Estimated H-index: 1
(University of Adelaide),
Shu-Biao Wu15
Estimated H-index: 15
(UNE: University of New England (United States))
+ 0 AuthorsJuliet Roberts2
Estimated H-index: 2
(UNE: University of New England (United States))
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Abstract
Background Eggshell formation takes place in the shell gland of the oviduct of laying hens. The eggshell is rich in calcium and various glycoproteins synthesised in the shell gland. Although studies have identified genes involved in eggshell formation, little is known about the regulation of genes in the shell gland particularly in a temporal manner. The current study investigated the global gene expression profile of the shell gland of laying hens at different time-points of eggshell formation using RNA-Sequencing (RNA-Seq) analysis.
  • References (69)
  • Citations (2)
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References69
Newest
Published on Jan 1, 2018in Journal of Proteomics3.54
Bernhard Mlecnik15
Estimated H-index: 15
,
Jérôme Galon62
Estimated H-index: 62
(Paris V: Paris Descartes University),
Gabriela Bindea23
Estimated H-index: 23
(Paris V: Paris Descartes University)
The interpretation of high dimensional datasets resulting from genomic and proteomic experiments in a timely and efficient manner is challenging. ClueGO software is a Cytoscape App that extracts representative functional biological information for large lists of genes or proteins. The functional enrichment analysis is based on the latest publicly available data from multiple annotation and ontology resources that can be automatically accessed through ClueGO. Predefined settings for the selection...
Published on Dec 1, 2017in Scientific Reports4.01
Samiullah Khan1
Estimated H-index: 1
(University of Adelaide),
J. R. Roberts14
Estimated H-index: 14
(UNE: University of New England (Australia)),
Shu-Biao Wu15
Estimated H-index: 15
(UNE: University of New England (Australia))
Ten reference genes were investigated for normalisation of candidate target gene expression data in the shell gland and spleen of laying hens challenged with two strains of infectious bronchitis virus (IBV). Data were analysed with geNorm, NormFinder and BestKeeper, and a comprehensive ranking (geomean) was calculated. In the combined data set of IBV challenged shell gland samples, the comprehensive ranking showed TATA-box binding protein (TBP) and tyrosine 3-monooxygenase/tryptophan 5-monooxyge...
Published on Mar 21, 2017
Maureen Bain1
Estimated H-index: 1
Published on Jan 1, 2017in Poultry Science2.03
S. Samiullah5
Estimated H-index: 5
(UNE: University of New England (Australia)),
Amal Saleh Omar1
Estimated H-index: 1
(UNE: University of New England (Australia))
+ 1 AuthorsK. K. Chousalkar6
Estimated H-index: 6
(UNE: University of New England (Australia))
Published on Dec 1, 2016in BMC Genomics3.50
Edin Hamzic4
Estimated H-index: 4
(AU: Aarhus University),
Rikke B. Kjærup3
Estimated H-index: 3
(AU: Aarhus University)
+ 9 AuthorsBart Buitenhuis13
Estimated H-index: 13
(AU: Aarhus University)
Background Avian infectious bronchitis is a highly contagious disease of the upper-respiratory tract caused by infectious bronchitis virus (IBV). Understanding the molecular mechanisms involved in the interaction between innate and adaptive immune responses to IBV infection is a crucial element for further improvements in strategies to control IB. To this end, two chicken lines, selected for high (L10H line) and low (L10L line) serum concentration of mannose-binding lectin (MBL) were studied. In...
Published on Dec 1, 2016in Veterinary Research3.12
Xuefeng Qi3
Estimated H-index: 3
(NWAFU: Northwest A&F University),
Dan Tan3
Estimated H-index: 3
(NWAFU: Northwest A&F University)
+ 7 AuthorsJingyu Wang5
Estimated H-index: 5
(NWAFU: Northwest A&F University)
This study aimed to determine the mechanism by which H9N2 avian influenza virus (AIV) affects eggshell quality. Thirty-week-old specific pathogen free egg-laying hens were inoculated with the chicken-origin H9N2 AIV strain (A/Chicken/shaanxi/01/2011) or with inoculating media without virus by combined intraocular and intranasal routes. The time course for the appearance of viral antigen and tissue lesions in the oviduct was coincident with the adverse changes in egg production in the infected he...
Published on Apr 20, 2015in Nucleic Acids Research11.15
Matthew E. Ritchie30
Estimated H-index: 30
(University of Melbourne),
Belinda Phipson17
Estimated H-index: 17
(Royal Children's Hospital)
+ 4 AuthorsGordon K. Smyth73
Estimated H-index: 73
(University of Melbourne)
limma is an R/Bioconductor software package that provides an integrated solution for analysing data from gene expression experiments. It contains rich features for handling complex experimental designs and for information borrowing to overcome the problem of small sample sizes. Over the past decade, limma has been a popular choice for gene discovery through differential expression analyses of microarray and high-throughput PCR data. The package contains particularly strong facilities for reading...
Published on Apr 1, 2015in Gene2.64
S. Bagés1
Estimated H-index: 1
(University of Lleida),
J. Estany1
Estimated H-index: 1
(University of Lleida)
+ 1 AuthorsR.N. Pena1
Estimated H-index: 1
(University of Lleida)
Accurate normalization of data is required to correct for different efficiencies and errors during the processing of samples in reverse transcription PCR analysis. The chicken is one of the main livestock species and its genome was one of the first reported and used in large scale transcriptomic analysis. Despite this, the chicken has not been investigated regarding the identification of reference genes suitable for the quantitative PCR analysis of growth and fattening genes. In this study, five...
Published on Jan 1, 2015
A. L. Johnson34
Estimated H-index: 34
(PSU: Pennsylvania State University)
The avian lineage evolved from ancestral, oviparous amniotes that include reptiles and the archosaurs (therapod dinosaurs and crocodilians). Oviparity represents the ancestral form of reproduction in all archosaurs, and it is the only form of reproduction utilized by birds. Whereas all modern crocodilians have two functional ovaries, only the left ovary is functional in the majority of avian species. Significantly, avian fossils from two species of an extinct lineage (enantiornithine birds of th...
Published on Jan 1, 2015in Journal of Proteomics3.54
Pauline Marie4
Estimated H-index: 4
(INRA: Institut national de la recherche agronomique),
Valérie Labas28
Estimated H-index: 28
(François Rabelais University)
+ 4 AuthorsJ. Gautron27
Estimated H-index: 27
(INRA: Institut national de la recherche agronomique)
Abstract Gallus gallus eggshell is a bioceramic composed of 95% calcium carbonate in calcitic form and 3.5% extracellular organic matrix. The calcification process occurs in the uterine fluid where biomineralization follows a temporal sequence corresponding to the initiation, growth and termination stages of crystal growth. Eggshell texture and its ultrastructure are regulated by organic matrix proteins, which control mineralization process and influence the eggshell biomechanical properties. We...
Cited By2
Newest
Published on Jun 1, 2019in Animal2.03
Zengkui Lu2
Estimated H-index: 2
,
Mingxing Chu + 5 AuthorsCaihong Wei5
Estimated H-index: 5
With the intensified and large-scale development of sheep husbandry and global warming, sheep heat stress has become an increasingly important issue. However, little is known about the molecular mechanisms related to sheep responses to heat stress. In this study, transcriptomic analysis of liver tissues of sheep in the presence and absence of heat stress was conducted, with the goal of identifying genes and pathways related to regulation when under such stress. After a comparison with the sheep ...
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