Original paper
Tandem Affinity Purification and Mass Spectrometry (TAP‐MS) for the Analysis of Protein Complexes
Abstract
Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP) primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version...
Paper Details
Title
Tandem Affinity Purification and Mass Spectrometry (TAP‐MS) for the Analysis of Protein Complexes
Published Date
Feb 1, 2019
Volume
96
Issue
1
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Notes
History