Direct Observation of DNA Target Searching and Cleavage by CRISPR-Cas12a
Abstract
Cas12a (also called Cpf1) is a representative type V-A CRISPR effector RNA-guided DNA endonuclease, which provides an alternative to type II CRISPR-Cas9 for genome editing. Previous studies have revealed that Cas12a has unique features distinct from Cas9, but the detailed mechanisms of target searching and DNA cleavage by Cas12a are still unclear. Here, we directly observe this entire process by using single-molecule fluorescence assays to study...
Paper Details
Title
Direct Observation of DNA Target Searching and Cleavage by CRISPR-Cas12a
Published Date
Feb 1, 2019
Journal
Volume
116
Issue
3
Pages
211a - 211a
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