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Cryocrystallography is an indispensable technique that is routinely used for single-crystal X-ray diffraction data collection at temperatures near 100 K, where radiation damage is mitigated. Modern procedures and tools to cryoprotect and rapidly cool macromolecular crystals with a significant solvent fraction to below the glass-transition phase of water are reviewed. Reagents and methods to help prevent the stresses that damage crystals when flash-cooling are described. A method of using isopent...
Published on Oct 20, 2009
Bernhard Rupp30
Estimated H-index: 30
PART I: FROM SEQUENCE TO CRYSTALS 1. Introduction: Preparing Your Study 2. Protein Structure 3. Protein Crystallization 4. Proteins for Crystallography PART II: FUNDAMENTALS OF PROTEIN CRYSTALLOGRAPHY 5. Crystal Geometry 6. Diffraction Basics 7. Statistics and Probability in Crystallography PART III: FROM CRYSTALS TO DATA 8. Instrumentation and Data Collection PART IV: DETERMINING YOUR STRUCTURE 9. Reconstruction of Electron Density and the Phase Problem 10. Experimental Phasing 11. Non-Crystall...
Published on Nov 1, 2004in Methods3.78
J.W. Pflugrath1
Estimated H-index: 1
Abstract Cryocrystallography is routinely used in macromolecular crystallography laboratories. The main advantage of X-ray diffraction data collection near 100 K is that crystals display much less radiation damage than seen at room temperature. Techniques and tools are described to facilitate cryoprotecting and flash-cooling crystals for data collection.
Published on Aug 1, 2003
Gregory A. Petsko83
Estimated H-index: 83
,
Dagmar Ringe65
Estimated H-index: 65
1. From Sequence to Structure 2. From Structure to Function 3. Control of Protein Function 4. From Sequence to Function: Case Studies in Structural and Functional Genomics 5. Structure Determination
Published on Jan 1, 1995
David B. Weiner79
Estimated H-index: 79
,
William V. Williams26
Estimated H-index: 26
Chemical Approaches to Rational Drug Design Design, Synthesis and Antitumor Activity of an Inhibitor of Ribonucleotide Reductase, J.R. McCarthy and P.S. Sunkara. The Development of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Inhibitors, A. Stern. The Development of Potent Angiotensin II Receptor Antagonists Using a Novel Peptide Pharmacophore Model, R.M. Keenan, J. Weinstock, J.C. Hempel, J.M. Samanen, D.T. Hill, N. Aiyar, E.H. Ohlstein, and R.M. Edwards. Endothelin Structure and D...
Published on Aug 1, 1992in Journal of Crystal Growth1.57
Alexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside)
Abstract Because the variable set is so extensive over which successful crystallization conditions must be sought, and because the amount of protein available is often severely limiting, it is essential to conduct as efficient and economical a series of initial trials as possible. One approach to this problem is to set out a selection of parallel trials using a minimum quantity of protein to identify as quickly as possible the type of precipitant, pH, and temperature likely to yield useful resul...
Published on Sep 1, 1989in Journal of Molecular Biology5.07
Stanley Koszelak12
Estimated H-index: 12
(UCR: University of California, Riverside),
James Allan Dodds1
Estimated H-index: 1
(UCR: University of California, Riverside),
Alexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside)
Abstract Satellite tobacco mosaic virus (STMV), a small T = 1 icosahedral plant virus, has been crystallized in a form suitable for high-resolution X-ray diffraction analysis. The crystals, which diffract to better than 2.5 A resolution, are of space group I 222 and have unit cell dimensions of a = 176 A , b = 192 A and c = 205 A . The centers of the virus particles occupy 222 symmetry points in the unit cell and one quarter of the virus particle constitutes the asymmetric unit, which is therefo...
Published on Jun 1, 1987in Journal of Molecular Biology5.07
Alexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside),
Frances Jurnak25
Estimated H-index: 25
(UCR: University of California, Riverside)
+ 1 AuthorsSarjeet S. Gill43
Estimated H-index: 43
(UCR: University of California, Riverside)
Abstract Crystals suitable for high resolution X-ray diffraction analysis have been reproducibly grown of the 24,000 M r protein insect toxin from Bacillus thuringiensis . This protein, which demonstrates substantial insecticidal activity by inserting into phospholipid membranes, crystallizes as long square needles from polyethylene glycol 4000 at neutral pH. The crystals are of space group P 4 1 and have cell dimensions of a = b = 33 A and c = 235 A , which suggests to us a predominantly helica...
Published on Sep 1, 1984in Plant Physiology6.30
Robert Morrison5
Estimated H-index: 5
,
Gregory Delozier1
Estimated H-index: 1
+ 1 AuthorsAlexander McPherson53
Estimated H-index: 53
Parallel biochemical and crystallographic studies have been carried out on the protein concanavalin B from Jack Bean ( Canavalis ensiformis ). The studies show the protein to be a monomer of 33,000 daltons with unexceptional amino acid composition and no covalently bound carbohydrate. The molecule contains a single, firmly bound zinc ion and we present evidence that the protein specifically binds nucleotide coenzymes including at least NADPH and flavin mononucleotide. An electron density map of ...
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