Autofluorescence Suppression by Optically Controlling Dark States of Photoswitchable Fluorescent Proteins on Commercial Microscopes

Inhomogeneous autofluorescence of live cells generates false positive signals that confound fluorescence imaging of dim objects. The ability to optically control the photophysical bright and dark states of photoswitchable fluorescent proteins (PS-FPs) provides an alternative means to suppress the same emission-wavelength autofluorescence. Previous demonstration of synchronously amplified fluorescence image recovery (SAFIRe) shows >10-fold...