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Biosensor for the oxidative stress biomarker glutathione based on SAM of cobalt phthalocyanine on a thioctic acid modified gold electrode

Published on Apr 1, 2019in Journal of Solid State Electrochemistry2.531
· DOI :10.1007/s10008-018-04191-4
M. N. Abbas16
Estimated H-index: 16
,
Ayman Ali Saeed4
Estimated H-index: 4
+ 4 AuthorsBaljit Singh21
Estimated H-index: 21
(Institute of Technology, Tallaght)
Abstract
Self-assembled monolayer (SAM) of cobalt teraaminophthalocyanine (CoTAPc) was developed on thioctic acid (TA) dithiol modified gold electrode and electrochemically evaluated as a glutathione (GSH) selective biosensor. The CoTAPc-TA-Au modified electrode was developed by the covalent immobilization of the CoTAPc as the electrochemical mediator onto previously prepared gold electrode modified with TA (TA-Au) via amid bond formation with the carboxylic group of TA, producing well-organized SAM of the mediator. For comparison, another electrode modified with 3-mercaptopropionic acid (MPA) as a monothiol linker instead of TA was similarly prepared. The electrode surface modification was characterized using SEM, AFM, CV, and EIS. The contact angle measurements of the surface confirmed the formation of CoTAPc SAM on both TA and MPA modified electrodes. The CoTAPc-TA-Au modified electrode showed enhanced catalytic activity for GSH oxidation compared to that of CoTAPc-MPA-Au, indicating that the TA dithiol allowed for more coverage of the catalyst layer on the electrode surface with stronger binding. The experimental parameters controlling the voltammetric processes like scan rate and pH of sample solution were optimized. Using DPV technique, the proposed sensor exhibited a linear response of oxidation peak current vs. GSH concentration, over the concentration range between 10 and 100 μmol L−1 with a LOD of 1.5 μmol L−1 for the CoTAPc-TA-Au modified electrode compared to 5.5 μmol L−1 GSH, for the CoTAPc-MPA-Au electrode. The proposed sensor was utilized for detection of glutathione in some hemolyzed blood samples.
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References54
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Abstract The development of reliable and cost-affordable electrochemical platform for rapid, sensitive, and selective detection of biological thiols has been sought after for various applications including clinical diagnosis and food monitoring. In this study, a novel disposable l -cysteine (CySH) sensor was developed based on screen-printed gold electrode (SPAuE) electrodeposited with copper. The constant potential electrodeposition was carried out at −0.4 V (versus an Ag pseudo-reference) for ...
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Abstract This article presents a highly sensitive and specific new sensing platform for cysteine determination. For the fabrication of electrochemical sensor, reduced graphene oxide-β-cyclodextrin-platinum nanocomposite (GR/CD/Pt) was prepared and surface of screen-printed electrodes were bulk modified with this nanocomposite which exhibited excellent electrocatalytic activity towards the sensing of cysteine. The GR/CD/Pt was characterized using SEM, TEM, AFM, FT-IR, TGA. The electrochemical beh...
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J. Iniesta thanks the programme Salvador de Madariaga from the Ministerio de Economia y Competitividad, grant number PRX14/00363. This work has been also financially supported by the MICINN-FEDER (Spain) through the projects CTQ2013-48280-C3-3-R.
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Electrochemical detection of nitrite was achieved via electrodeposition of gold nanoparticles (AuNPs) onto glassy carbon electrodes, followed by 3-mercaptopropionic acid (MPA) self-assembly, enabling attachment of an iron(III) monoamino-phthalocyanine (FeMAPc) catalyst via amide bond formation. The use of scanning electron microscopy, energy dispersive X-ray spectroscopy and ultraviolet-visible spectroscopy realised surface characterisation while cyclic voltammetry and electrochemical impedance ...
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