Use of Protease from Seabass Pyloric Caeca in Combination with Repeated Freeze–Thawing Cycles Increases the Production Efficiency of Virgin Coconut Oil

Virgin coconut oil (VCO) is separated from coconut milk using partially purified protease from seabass pyloric caeca (PPSP) in combination with different treatments including micro‐fluidization, chill–thawing, and freeze–thawing. The lowest VCO yield is obtained for coconut milk homogenized at 4000 psi, followed by hydrolysis using PPSP at the level of 5 units g −1 proteins. Conversely, the higher VCO yield is obtained from non‐homogenized and...