Original paper
Artificial Restriction DNA Cutter Using Nuclease S1 for Site‐Selective Scission of Genomic DNA
Abstract
By combining a pair of pseudo‐complementary peptide nucleic acids (pcPNAs) with S1 nuclease, a novel tool to cut DNA at a predetermined site can be obtained. Complementary pcPNAs invade the DNA duplex and base pair to each strand of a target site, creating single‐stranded regions that are cleaved by S1 nuclease. The scission site can be freely modulated by the design of pcPNAs. This method can be used to cleave a single site in the human genome....
Paper Details
Title
Artificial Restriction DNA Cutter Using Nuclease S1 for Site‐Selective Scission of Genomic DNA
Published Date
Feb 5, 2019
Volume
76
Issue
1
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History