Immobilization of antimicrobial and anti-quorum sensing enzymes onto GMA-grafted poly(vinyl chloride) catheters

Published on Mar 1, 2019in International Journal of Pharmaceutics4.213
· DOI :10.1016/j.ijpharm.2018.12.075
Alejandro Costoya4
Estimated H-index: 4
(University of Santiago de Compostela),
Luis E. Velázquez Becerra1
Estimated H-index: 1
(UNAM: National Autonomous University of Mexico)
+ 6 AuthorsCarmen Alvarez-Lorenzo51
Estimated H-index: 51
(University of Santiago de Compostela)
Abstract Catheter-associated infections still represent a challenging thread because of the likelihood of biofilm formation. The aim of this work was the surface modification of catheters to immobilize lysozyme and acylase under mild conditions while preserving antimicrobial and anti-quorum sensing performances. Glycidyl methacrylate (GMA) was grafted onto poly(vinyl chloride) (PVC) catheters by a pre-irradiation method. The effects of monomer concentration, pre-irradiation dose, reaction time, monomer concentration and reaction temperature were investigated. The grafting process was monitored using FTIR-ATR spectroscopy, differential scanning calorimetry, thermogravimetric analysis, and swelling data. Lysozyme was directly immobilized onto PVC-g-GMA maintaining the hydrolytic activity, which hindered Staphylococcus aureus adhesion. For acylase immobilization, the PVC-g-GMA catheters were reacted with ethylenediamine and glutaraldehyde in order to facilitate acylase covalent binding. Free acylase in solution demonstrated notably capability to act as quorum sensing inhibitor, as observed using Chromobacterium violaceum as biosensor, by degrading a wide variety of acylated homoserine lactones (AHLs), including those produced by Pseudomonas aeruginosa and Acinetobacter baumannii. Acylase-immobilized PVC-g-GMA catheters were challenged against degradation of AHLs and the activity monitored using both the biosensor and HPLC-MS. Relevantly, the functionalized catheters completely degraded all tested AHL signals, opening new ways of preventing biofilm formation on medical devices.
  • References (65)
  • Citations (0)
📖 Papers frequently viewed together
2 Citations
2 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Celia Mayer (University of Santiago de Compostela)H-Index: 7
#2Andrea Muras (University of Santiago de Compostela)H-Index: 6
Last. Ana Otero (University of Santiago de Compostela)H-Index: 28
view all 6 authors...
Acinetobacter baumannii presents a typical luxI/luxR quorum sensing (QS) system (abaI/abaR) but the acyl-homoserine lactone (AHL) signal profile and factors controlling the production of QS signals in this species have not been determined yet. A very complex AHL profile was identified for A. baumannii ATCC17978 as well as for A. nosocomialis M2, but only when cultivated under static conditions, suggesting that surface or cell-to-cell contact is involved in the activation of the QS genes. The ana...
4 CitationsSource
Pseudomonas aeruginosa is the predominant pathogen in pulmonary infections associated with cystic fibrosis. Quorum sensing (QS) systems regulate the production of virulence factors and play an important role in the establishment of successful P. aeruginosa infections. Inhibition of the QS system (termed quorum quenching) renders the bacteria avirulent thus serving as an alternative approach in the development of novel antibiotics. Quorum quenching in Gram negative bacteria can be achieved by pre...
3 CitationsSource
#1Andrea MurasH-Index: 6
#2Mario López-PérezH-Index: 17
Last. Ana OteroH-Index: 28
view all 6 authors...
There is increasing evidence being accumulated regarding the importance of N-acyl homoserine lactones (AHL)-mediated quorum-sensing (QS) and quorum-quenching (QQ) processes in the marine environment, but in most cases, data has been obtained from specific microhabitats, and subsequently little is known regarding these activities in free-living marine bacteria. The QS and QQ activities among 605 bacterial isolates obtained at 90 and 2000 m depths in the Mediterranean Sea were analyzed. Additional...
10 CitationsSource
The aim of this Research Article is to present three different techniques of poly(sodium styrene sulfonate) (polyNaSS) covalent grafting onto titanium (Ti) surfaces and study the influence of their architecture on biological response. Two of them are “grafting from” techniques requiring an activation step either by thermal or UV irradiation. The third method is a “grafting to” technique involving an anchorage molecule onto which polyNaSS synthesized by reversible addition–fragmentation chain tra...
6 CitationsSource
#1Ivette Zuñiga-Zamorano (UNAM: National Autonomous University of Mexico)H-Index: 1
Last. Emilio Bucio (UNAM: National Autonomous University of Mexico)H-Index: 22
view all 6 authors...
Abstract Radiation-grafting of pH-responsive methacrylic acid (MAA) onto poly(vinyl chloride) (PVC) was carried out by the pre-irradiation method using gamma rays, which demonstrated to be an efficient and fast procedure for obtaining PVC-g-MAA copolymers. The influence of preparation conditions, such as absorbed dose, monomer concentration, reaction time, and reaction temperature on the grafting yield was studied. The grafting of MAA onto PVC catheters was confirmed by means of Fourier transfor...
3 CitationsSource
Pseudomonas aeruginosa and Acinetobacter baumannii are two of the main bacteria responsible for nosocomial infections; both organisms are resistant to several classes of antibiotics making their infections very difficult to treat. Moreover, they possess a remarkable ability to form biofilms, which further enhances their antimicrobial resistance. Both organisms coordinate their formation of biofilms and their expression of virulence factors through quorum sensing, a system that regulates gene exp...
6 CitationsSource
#1Jeongjoon Lee (KU: Korea University)H-Index: 1
#2In-Seon Lee (KU: Korea University)H-Index: 30
Last. Jungbae KimH-Index: 36
view all 6 authors...
Acylase (AC) was immobilized and stabilized on carboxylated polyaniline nanofibers (cPANFs) for the development of antifouling nanobiocatalysts with high enzyme loading and stability. AC was immobilized via three different approaches: covalent attachment (CA), enzyme coating (EC), and magnetically separable enzyme precipitate coating (Mag-EPC). The enzyme activity per unit weight of cPANFs with Mag-EPC was 75 and 300 times higher than that of those with CA and EC, respectively, representing impr...
24 CitationsSource
#1Priyadarshini Singha (UGA: University of Georgia)H-Index: 5
#2Jason Locklin (UGA: University of Georgia)H-Index: 40
Last. Hitesh Handa (UGA: University of Georgia)H-Index: 20
view all 3 authors...
Abstract More than 75% of hospital-acquired or nosocomial urinary tract infections are initiated by urinary catheters, which are used during the treatment of 15–25% of hospitalized patients. Among other purposes, urinary catheters are primarily used for draining urine after surgeries and for urinary incontinence. During catheter-associated urinary tract infections, bacteria travel up to the bladder and cause infection. A major cause of catheter-associated urinary tract infection is attributed to...
53 CitationsSource
#1Navdeep Grover (CU: University of Colorado Boulder)H-Index: 8
#2Joseph G. Plaks (CU: University of Colorado Boulder)H-Index: 6
Last. Joel L. Kaar (CU: University of Colorado Boulder)H-Index: 20
view all 6 authors...
Due to the prevalence of biofilm-related infections, which are mediated by bacterial quorum sensing, there is a critical need for materials and coatings that resist biofilm formation. We have developed novel anti-biofilm coatings that disrupt quorum sensing in surface-associated bacteria via the immobilization of acylase in polyurethane films. Specifically, acylase from Aspergillus melleus was covalently immobilized in biomedical grade polyurethane coatings via multipoint covalent immobilization...
14 CitationsSource
#1Luisa Islas (UNAM: National Autonomous University of Mexico)H-Index: 2
#2Juan-Carlos Ruiz (UAM: Universidad Autónoma Metropolitana)H-Index: 14
Last. Guillermina Burillo (UNAM: National Autonomous University of Mexico)H-Index: 23
view all 5 authors...
Abstract Poly(vinyl chloride) (PVC) urinary catheters were modified with either a single or binary graft of acrylic acid (AAc) and/or poly(ethylene glycol) methacrylate (PEGMA) using gamma-radiation from 60 Co to obtain PVC- g -AAc, PVC- g -PEGMA, [PVC- g -AAc]- g -PEGMA, and [PVC- g -PEGMA]- g -AAc copolymers. The outer and inner surfaces of the modified catheters were characterized using scanning electron microscopy (SEM), confocal laser microscopy (CLM) and X-ray photoelectron spectroscopy (X...
9 CitationsSource
Cited By0
#1Qingchen Yang (Huada: Beijing University of Chemical Technology)
#2Yuzheng Xia (Huada: Beijing University of Chemical Technology)H-Index: 4
Last. Lei Lei (UNSW: University of New South Wales)H-Index: 8
view all 8 authors...
Abstract In this paper, crosslinked polyacrylate latex with tertiary amine groups (ACLN) and base latex without tertiary amine groups (ACL) were prepared by emulsion polymerization using butyl acrylate as the monomer and 1,4-butanediol dimethacrylate as the crosslinker. Composite resins of polyvinyl chloride (PVC), ACL/PVC and ACLN/PVC, were prepared by suspension polymerization of vinyl chloride in a 20 L high-pressure reactor by adding ACL and ACLN as modifiers. The inner pressure of the react...
#1Hector Magaña (UABC: Autonomous University of Baja California)H-Index: 3
#2Claudia D. Becerra (UABC: Autonomous University of Baja California)
Last. Jose M. Cornejo-Bravo (UABC: Autonomous University of Baja California)H-Index: 14
view all 8 authors...
Silicone rubber (SR) is a material used for medical procedures, with a common example of its application being in implants for cosmetic or plastic surgeries. It is also an essential component for the development of medical devices. SR was functionalized with the polymeric prodrug of poly(2-methacryloyloxy-benzoic acid) (poly(2MBA)) to render the analgesic anti-inflammatory drug salicylic acid by hydrolysis. The system was designed by functionalizing SR films (0.5 cm × 1 cm) with a direct graftin...
Last. Ernesto Rivera (UNAM: National Autonomous University of Mexico)H-Index: 16
view all 6 authors...
Polysulfone (PSU) film and N-vinylimidazole (VIM) were used to obtain grafted membranes with high hydrophilic capacity. The grafting process was performed by gamma irradiation under two experiments: (1) different irradiation doses (100–400 kGy) and VIM 50% solution; (2) different concentration of grafted VIM (30–70%) and 300 kGy of irradiation dose. Characteristics of the grafted membranes were determined by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), cont...
#1Dan-ni Huang (ZJU: Zhejiang University)H-Index: 1
#2Jing WangH-Index: 1
Last. Jian Ji (ZJU: Zhejiang University)H-Index: 54
view all 4 authors...
Pathogenic microbial biofilms readily form on implantable medical devices or human tissues have posed a great threat to worldwide healthcare. Hopes are focused on preventive strategies of biofilms, leaving a thought-provoking question: how to tackle the problem of established biofilms? In this review, we briefly summarize the functionalized biomaterials to combat biofilm and highlight current approaches to eradicate pre-existed biofilms. We believe that all of these strategies, alone or in combi...