PCR Assays Based on invA Gene Amplification are not Reliable for Salmonella Detection

Background: Salmonella surveillance relies on invA polymerase chain reaction (PCR) assays for the rapid detection of Salmonella; however, false-positive results have been reported using this method. Objectives: To evaluate the performance and specificity of the published and validated PCR protocols targeting invA gene for the detection of Salmonella. Methods: The performance and specificity of 11 different PCR primer sets were evaluated using...