Match!

Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro

Published on Jan 10, 2019in Nature Communications11.88
· DOI :10.1038/s41467-018-08003-1
Carola Fischer1
Estimated H-index: 1
(LMU: Ludwig Maximilian University of Munich),
Hendrik Milting26
Estimated H-index: 26
(RUB: Ruhr University Bochum)
+ 11 AuthorsAndreas Dendorfer31
Estimated H-index: 31
(LMU: Ludwig Maximilian University of Munich)
Abstract
In vitro models incorporating the complexity and function of adult human tissues are highly desired for translational research. Whilst vital slices of human myocardium approach these demands, their rapid degeneration in tissue culture precludes long-term experimentation. Here, we report preservation of structure and performance of human myocardium under conditions of physiological preload, compliance, and continuous excitation. In biomimetic culture, tissue slices prepared from explanted failing human hearts attain a stable state of contractility that can be monitored for up to 4 months or 2000000 beats in vitro. Cultured myocardium undergoes particular alterations in biomechanics, structure, and mRNA expression. The suitability of the model for drug safety evaluation is exemplified by repeated assessment of refractory period that permits sensitive analysis of repolarization impairment induced by the multimodal hERG-inhibitor pentamidine. Biomimetic tissue culture will provide new opportunities to study drug targets, gene functions, and cellular plasticity in adult human myocardium.
  • References (45)
  • Citations (3)
References45
Newest
#1Yun Qiao (GW: George Washington University)H-Index: 2
#2Quan Dong (GW: George Washington University)H-Index: 2
Last.Zhenyu Li (GW: George Washington University)H-Index: 14
view all 11 authors...
#1Kacey Ronaldson-Bouchard (Columbia University)H-Index: 3
#2Stephen P. Ma (Columbia University)H-Index: 3
Last.Gordana Vunjak-Novakovic (Columbia University)H-Index: 106
view all 10 authors...
#1Filippo Perbellini (NIH: National Institutes of Health)H-Index: 6
#2Samuel A. Watson (NIH: National Institutes of Health)H-Index: 3
Last.André SimonH-Index: 1
view all 16 authors...
#1Anastasia Korolj (U of T: University of Toronto)H-Index: 8
#2Erika Yan Wang (U of T: University of Toronto)H-Index: 3
Last.Milica RadisicH-Index: 50
view all 4 authors...
#1Thomas Seidel (FAU: University of Erlangen-Nuremberg)H-Index: 9
#2Sutip Navankasattusas (FAU: University of Erlangen-Nuremberg)H-Index: 6
Last.Craig H. Selzman (FAU: University of Erlangen-Nuremberg)H-Index: 38
view all 12 authors...
#1Nikolina Jovancevic (RUB: Ruhr University Bochum)H-Index: 7
#2Andreas Dendorfer (LMU: Ludwig Maximilian University of Munich)H-Index: 31
Last.Judith Semmler (University of Cologne)H-Index: 3
view all 15 authors...
View next paperSpatial and Electrical Factors Regulating Cardiac Regeneration and Assembly