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Assessing the utilization of high‐resolution 2‐field HLA typing in solid organ transplantation

Published on Jul 1, 2019in American Journal of Transplantation7.163
· DOI :10.1111/ajt.15258
Yanping Huang2
Estimated H-index: 2
(Children's Hospital of Philadelphia),
Anh Dinh2
Estimated H-index: 2
(Children's Hospital of Philadelphia)
+ 14 AuthorsDimitri S. Monos34
Estimated H-index: 34
(UPenn: University of Pennsylvania)
Abstract
  • References (27)
  • Citations (2)
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References27
Newest
#1June A. Jones (Johns Hopkins University)H-Index: 1
#2Annette M. Jackson (Johns Hopkins University)H-Index: 22
Last. Maria Bettinotti (JHUSOM: Johns Hopkins University School of Medicine)H-Index: 4
view all 4 authors...
Aim Use of HLA P groups for reporting purposes and DSA assessments for potential donors has been steadily increasing. P groups for HLA class II encompass exon2 sequences and capture the polymorphic rich peptide binding domain that serves as the primary target for alloantibody. Nevertheless, we have observed multiple sera exhibiting HLA-DQA1 or HLA-DPB1 reactivity to polymorphic sites outside of exon 2 and confirmed this reactivity using cell adsorptions. Methods HLA antibodies were assessed usin...
1 CitationsSource
#1Anat R. Tambur (NU: Northwestern University)H-Index: 25
#2Patricia Campbell (U of A: University of Alberta)H-Index: 21
Last. Peter Nickerson (UM: University of Manitoba)H-Index: 45
view all 42 authors...
28 CitationsSource
#1Allyson Hart (UMN: University of Minnesota)H-Index: 9
#2Jodi M. Smith (UW: University of Washington)H-Index: 38
Last. A. K. Israni (UMN: University of Minnesota)H-Index: 34
view all 11 authors...
Data from 2016 show ongoing positive trends in short- and long-term allograft survival, and a decrease in the number of active listed candi- dates for the first time in more than a decade, with a concomitant in- crease in deceased donor kidney transplants. Transplant rates that had changed dramatically for some groups after implementation of the new kidney allocation system in 2014 are stabilizing, allowing for evaluation of new steady states and trends. Many challenges remain in adult kid- ney ...
140 CitationsSource
AbstractThe evolution of antibody detection from cell- to bead-based technology has positively impacted the ability to allocate organs in a safe and timely manner. The devil, of course, is in the details that delineate how these assays are performed and applied and to recognize that while there have
12 CitationsSource
#1Matthew P Sypek (University of Melbourne)H-Index: 4
#2Joshua Y. Kausman (University of Melbourne)H-Index: 8
Last. Peter D. Hughes (University of Melbourne)H-Index: 15
view all 4 authors...
Rapid changes in tissue-typing technology, including the widespread availability of highly specific molecular typing methods and solid-phase assays for the detection of allele-specific anti-HLA antibodies, make it increasingly challenging to remain up to date with developments in organ matching. Terms such as epitopes and eplets abound in the transplantation literature, but often it can be difficult to see what they might mean for the patient awaiting transplantation. In this review, we provide ...
5 CitationsSource
#1Vasilis Kosmoliaptsis (University of Cambridge)H-Index: 17
#2D. H. Mallon (University of Cambridge)H-Index: 8
Last. Craig J. Taylor (University of Cambridge)H-Index: 34
view all 6 authors...
This study was supported by the Cambridge NIHR Biomedical Research Centre and the NIHR Blood and Transplant Research Unit in Organ Donation and Transplantation at the University of Cambridge in collaboration with Newcastle University and in partnership with NHS Blood and Transplant (NHSBT). The views expressed are those of the authors and not necessarily those of the NHS, the NIHR, the Department of Health or NHSBT . VK was supported by an Academy of Medical Sciences Grant and an Evelyn Trust Gr...
34 CitationsSource
#1Jamie L. Duke (Children's Hospital of Philadelphia)H-Index: 6
#2Curt Lind (Children's Hospital of Philadelphia)H-Index: 11
Last. Dimitri S. Monos (UPenn: University of Pennsylvania)H-Index: 34
view all 13 authors...
This study presents performance specifications of an in-house developed human leukocyte antigen (HLA) typing assay using next-generation sequencing (NGS) on the Illumina MiSeq platform. A total of 253 samples, previously characterized for HLA-A, -B, -C, -DRB1 and -DQB1 were included in this study, which were typed at high-resolution using a combination of Sanger sequencing, sequence-specific primer (SSP) and sequence-specific oligonucleotide probe (SSOP) technologies and recorded at the two-fiel...
40 CitationsSource
#1Sebastiaan Heidt (LUMC: Leiden University Medical Center)H-Index: 17
#2Marian D. Witvliet (LUMC: Leiden University Medical Center)H-Index: 14
Last. Frans H.J. Claas (LUMC: Leiden University Medical Center)H-Index: 61
view all 4 authors...
Abstract In 2014, the Eurotransplant Acceptable Mismatch (AM) program celebrated its 25th anniversary. The AM program was initiated to enhance transplantation of highly sensitized patients awaiting a renal transplant within the Eurotransplant region. Unlike the regular renal transplant allocation, in which the histocompatibility parameters consist of the degree of compatibility with the patient's human leucocyte antigen (HLA) type and the absence of unacceptable antigens, the AM program is based...
43 CitationsSource
#1Patrick M. Sullivan (Children's Hospital Los Angeles)H-Index: 5
#2Paul Warner (Puget Sound Blood Center)H-Index: 8
Last. Yuk M. Law (Seattle Children's)H-Index: 19
view all 7 authors...
Background Although evidence links HLA allele mismatching to worse outcomes in pediatric heart transplantation, no studies to our knowledge have applied the quantification of structural HLA differences between donor and recipient to risk evaluation. We examine the association between molecular-level HLA mismatching and long-term graft loss in pediatric recipients of heart transplants. Methods HLA Matchmaker was used to quantify the number of mismatched class-specific HLA eplets among 4,851 heart...
16 CitationsSource
#1R. J. Duquesnoy (University of Pittsburgh)H-Index: 16
#2Malek Kamoun (UPenn: University of Pennsylvania)H-Index: 37
Last. Adriana Zeevi (University of Pittsburgh)H-Index: 63
view all 19 authors...
Division of Transplant Pathology, Thomas E. StarzlTransplantation Institute, University of Pittsburgh MedicalCenter, Pittsburgh, PACorresponding author: Rene J. Duquesnoy,Duquesnoyr@upmc.eduDefining HLA mismatch acceptability of organ trans-plant donors for sensitized recipients has traditionallybeenbasedonserologicallydefinedHLAantigens.Now,however, it is well accepted that HLA antibodiesspecifically recognize a wide range of epitopes presenton HLA antigens and that molecularly defined highresoluti...
45 CitationsSource
Cited By2
Newest
#1Olga TimofeevaH-Index: 17
#2René J. Alvarez (Thomas Jefferson University)H-Index: 2
Last. Meredith A. BriscoH-Index: 13
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Abstract Antibody-mediated rejection (AMR) of cardiac allografts mediated by anti-HLA Donor Specific Antibodies (DSA) is one of the major barriers to successful transplantation for the treatment of end-stage heart failure. Therapeutic plasma exchange (TPE) is a first-line treatment for pre-transplant desensitization. However, indications for treatment regimens and treatment end-points have not been well established. In this study, we investigated how sera dilutions could guide TPE regimens for e...
Source
#1Maureen Montgomery (UNC: University of North Carolina at Chapel Hill)H-Index: 1
#1Maureen C. Montgomery (UNC Health Care)H-Index: 2
Last. Eric T. Weimer (UNC: University of North Carolina at Chapel Hill)H-Index: 9
view all 4 authors...
Abstract Transplant centers are increasingly using virtual crossmatching (VXM) to evaluate recipient and donor compatibility. However, the current state of VXM fails to incorporate donor HLA expression in the assessment, despite numerous studies that demonstrated the impact of donor HLA expression on physical crossmatch outcomes. Whole-transcriptome sequencing (RNA-Seq) for HLA enables simultaneous determination of HLA genotyping and relative HLA expression. Ultimately the RNA-Seq needs to be fa...
Source
#1Anajane G. Smith (Fred Hutchinson Cancer Research Center)H-Index: 31
#2Shalini Pereira (UW: University of Washington)H-Index: 9
Last. Daniel E. Geraghty (Fred Hutchinson Cancer Research Center)H-Index: 55
view all 15 authors...
1 CitationsSource