A lentivirus-based system for Cas9/gRNA expression and subsequent removal by Cre-mediated recombination

Volume: 156, Pages: 79 - 84
Published: Mar 1, 2019
Abstract
A major concern of CRISPR and related genome engineering technologies is off-target mutagenesis from prolonged exposure to Cas9 and related editing enzymes. To help mitigate this concern we added a loxP site to the 3′-LTR of an HIV-based lentiviral vector capable of expressing Cas9/gRNA complexes in a wide variety of mammalian cell types. Transduction of susceptible target cells yields an integrated provirus that expresses the desired Cas9/gRNA...
Paper Details
Title
A lentivirus-based system for Cas9/gRNA expression and subsequent removal by Cre-mediated recombination
Published Date
Mar 1, 2019
Journal
Volume
156
Pages
79 - 84
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